Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1968 Nov;2(11):1326–1338. doi: 10.1128/jvi.2.11.1326-1338.1968

Cytoplasmic Structures Associated with an Arbovirus Infection: Loci of Viral Ribonucleic Acid Synthesis

Philip M Grimley 1, Irene K Berezesky 1, Robert M Friedman 1
PMCID: PMC375472  PMID: 5750316

Abstract

Unique cytoplasmic structures, herein designated as type I cytopathic vacuoles (CPV-I), are found in chick embryo cells early in the logarithmic phase of Semliki Forest virus replication. High resolution autoradiography demonstrated that the CPV-I are loci of 3H-uridine incorporation. This evidence correlates well with previous biochemical data and electron microscopy of the subcellular fractions active in Semliki Forest virus ribonucleic acid synthesis. Origin of the CPV-I within host cell cytoplasm is confirmed by the distribution of electron-dense tracer particles and sequential ultrastructural observations.

Full text

PDF
1326

Images in this article

1329Image
on p.1329
1329Image
on p.1329
1329Image
on p.1329
1330Image
on p.1330
1330Image
on p.1330
1330Image
on p.1330
1330Image
on p.1330
1331Image
on p.1331
1333Image
on p.1333
1333Image
on p.1333
1333Image
on p.1333
1334Image
on p.1334
1334Image
on p.1334
1334Image
on p.1334
1335Image
on p.1335

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson N. H., Tamm I. Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967 May;32(1):128–143. doi: 10.1016/0042-6822(67)90261-9. [DOI] [PubMed] [Google Scholar]
  2. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHENG P. Y. Purification, size, and morphology of a mosquitoborne animal virus, Semliki Forest virus. Virology. 1961 May;14:124–131. doi: 10.1016/0042-6822(61)90139-8. [DOI] [PubMed] [Google Scholar]
  4. Chain M. M., Doane F. W., McLean D. M. Morphological development of Chikungunya virus. Can J Microbiol. 1966 Oct;12(5):895–900. doi: 10.1139/m66-122. [DOI] [PubMed] [Google Scholar]
  5. Chambers R. W., Bowling M. C., Grimley P. M. Glutaraldehyde fixation in routine histopathology. Arch Pathol. 1968 Jan;85(1):18–30. [PubMed] [Google Scholar]
  6. Comings D. E., Kakefuda T. Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968 Apr 14;33(1):225–229. doi: 10.1016/0022-2836(68)90290-8. [DOI] [PubMed] [Google Scholar]
  7. DALES S., EGGERS H. J., TAMM I., PALADE G. E. ELECTRON MICROSCOPIC STUDY OF THE FORMATION OF POLIOVIRUS. Virology. 1965 Jul;26:379–389. doi: 10.1016/0042-6822(65)90001-2. [DOI] [PubMed] [Google Scholar]
  8. DALES S. The uptake and development of vaccinia virus in strain L cells followed with labeled viral deoxyribonucleic acid. J Cell Biol. 1963 Jul;18:51–72. doi: 10.1083/jcb.18.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eisenstein R., Battifora H., Axelrod E. Vitamin D-actinomycin D interactions in bone. An electron microscopic study of hypertrophy and hyperinvolution of osteoblasts. Lab Invest. 1966 Oct;15(10):1589–1600. [PubMed] [Google Scholar]
  10. Erlandson R. A., Babcock V. I., Southam C. M., Bailey R. B., Shipkey F. H. Semliki Forest virus in HEp-2 cell cultures. J Virol. 1967 Oct;1(5):996–1009. doi: 10.1128/jvi.1.5.996-1009.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FRASER K. B., CRAWFORD E. M. IMMUNOFLUORESCENT AND ELECTRON-MICROSCOPIC STUDIES OF POLYOMA VIRUS IN TRANSFORMATION REACTIONS WITH BHK21 CELLS. Exp Mol Pathol. 1965 Feb;76:51–65. doi: 10.1016/0014-4800(65)90023-7. [DOI] [PubMed] [Google Scholar]
  12. Friedman R. M., Berezesky I. K. Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J Virol. 1967 Apr;1(2):374–383. doi: 10.1128/jvi.1.2.374-383.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Friedman R. M. Protein synthesis directed by an arbovirus. J Virol. 1968 Jan;2(1):26–32. doi: 10.1128/jvi.2.1.26-32.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Friedman R. M. Replicative intermediate of an arbovirus. J Virol. 1968 Jun;2(6):547–552. doi: 10.1128/jvi.2.6.547-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Friedman R. M., Sonnabend J. A. Inhibition of interferon action by puromycin. J Immunol. 1965 Oct;95(4):696–703. [PubMed] [Google Scholar]
  17. Gosselin R. E. Kinetics of pinocytosis. Fed Proc. 1967 Jul-Aug;26(4):987–993. [PubMed] [Google Scholar]
  18. LWOFF A., HORNE R., TOURNIER P. A system of viruses. Cold Spring Harb Symp Quant Biol. 1962;27:51–55. doi: 10.1101/sqb.1962.027.001.008. [DOI] [PubMed] [Google Scholar]
  19. MORGAN C., HOWE C., ROSE H. M. Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J Exp Med. 1961 Jan 1;113:219–234. doi: 10.1084/jem.113.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. MUSSGAY M. GROWTH CYCLE OF ARBOVIRUSES IN VERTEBRATE AND ARTHROPOD CELLS. Prog Med Virol. 1964;6:193–267. [PubMed] [Google Scholar]
  21. MUSSGAY M., WEIBEL J. Electron microscopic and biological studies on the growth of Venezuelan equine encephalitis virus in KB cells. Virology. 1962 Jan;16:52–62. doi: 10.1016/0042-6822(62)90201-5. [DOI] [PubMed] [Google Scholar]
  22. Martin E. M., Sonnabend J. A. Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J Virol. 1967 Feb;1(1):97–109. doi: 10.1128/jvi.1.1.97-109.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McGee-Russell S. M., Gosztonyi G. Assembly of Semliki forest virus in brain. Nature. 1967 Jun 17;214(5094):1204–1206. doi: 10.1038/2141204a0. [DOI] [PubMed] [Google Scholar]
  24. OTA Z. ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS VIRUS IN PORCINE KIDNEY STABLE (PS) CELLS. Virology. 1965 Mar;25:372–378. doi: 10.1016/0042-6822(65)90057-7. [DOI] [PubMed] [Google Scholar]
  25. PELC S. R. The stripping-film technique of autoradiography. Int J Appl Radiat Isot. 1956 Nov;1(3):172–177. doi: 10.1016/0020-708x(56)90003-5. [DOI] [PubMed] [Google Scholar]
  26. Simard R., Bernhard W. Le phénomène de la ségrégation nucléolaire: spécificité d'action de certains antimétabolites. Int J Cancer. 1966 Sep 15;1(5):463–479. doi: 10.1002/ijc.2910010506. [DOI] [PubMed] [Google Scholar]
  27. YASUZUMI G., TSUBO I. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VIRUS. II. ELECTRON MICROSCOPE STUDIES OF NEURONS INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1965 Apr;12:304–316. doi: 10.1016/s0022-5320(65)80101-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES