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. 1968 Nov;2(11):1346–1352. doi: 10.1128/jvi.2.11.1346-1352.1968

Characterization of Intracellular Ribonucleic Acid Specific for the Murine Sarcoma-Leukemia Virus Complex

Nilambar Biswal 1, Michael B Grizzard 1,1, Robert M McCombs 1, Matilda Benyesh-Melnick 1
PMCID: PMC375474  PMID: 4302749

Abstract

Virus particles were continuously produced by a cell line (78A1) of rat embryo fibroblasts that had been transformed by the murine sarcoma-leukemia virus complex. Since most of the mature virions were found in the extracellular fluid and were not cell-associated, a measurable quantity of viral ribonucleic acid (RNA) could not be extracted from these cells. Cycloheximide, a protein inhibitor, was successfully used to accumulate viral RNA within the cells. This ribonuclease-sensitive RNA, with a sedimentation coefficient of 71S, had the same base composition as the high molecular weight RNA (S20,w = 71) isolated from purified virions released by the transformed cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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