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. 1968 Dec;2(12):1393–1399. doi: 10.1128/jvi.2.12.1393-1399.1968

Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3

R H Olsen a, Eleanor S Metcalf a, Carl Brandt a,1
PMCID: PMC375484  PMID: 5745508

Abstract

Restriction of Pseudomonas bacteriophage CB3 growth on some Pseudomonas aeruginosa hosts was studied. On restricting hosts, growth of this phage was severely inhibited below 32 C and hence was temperature-sensitive. Investigation of this phenomenon revealed that restricting hosts were not killed as a consequence of their infection under nonpermissive conditions. The ability of some hosts to restrict showed segregation in sexual crosses between restricting and nonrestricting hosts. However, the pattern of restriction among various hosts differed with the phage in question when other phages were compared with CB3. Temperature-shift experiments indicated that blockage of an early event in the phage lytic cycle occurred when restricting conditions were imposed on cells infected with CB3. This blockage could be eliminated by holding at permissive conditions until the cold-sensitive step was bypassed or by pulsing restricting cells for 5 min at 37 C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arber W. Host-controlled modification of bacteriophage. Annu Rev Microbiol. 1965;19:365–378. doi: 10.1146/annurev.mi.19.100165.002053. [DOI] [PubMed] [Google Scholar]
  2. BERTANI G., NICE S. J. Studies on lysogenesis. II. The effect of temperature on the lysogenization of Shigella dysenteriae with phage P1. J Bacteriol. 1954 Feb;67(2):202–209. doi: 10.1128/jb.67.2.202-209.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERTANI G., WEIGLE J. J. Host controlled variation in bacterial viruses. J Bacteriol. 1953 Feb;65(2):113–121. doi: 10.1128/jb.65.2.113-121.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benzer S. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344–354. doi: 10.1073/pnas.41.6.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen J. R., Saul S. H. A cold-sensitive mutant of bacteriophage T1. Virology. 1966 Jul;29(3):497–499. doi: 10.1016/0042-6822(66)90228-5. [DOI] [PubMed] [Google Scholar]
  6. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
  7. Dowell C. E. Cold-sensitive mutants of bacteriophage phi-X-174. I. A mutant blocked in the eclipse function at low temperature. Proc Natl Acad Sci U S A. 1967 Sep;58(3):958–961. doi: 10.1073/pnas.58.3.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EDGAR R. S., LIELAUSIS I. TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4D: THEIR ISOLATION AND GENETIC CHARACTERIZATION. Genetics. 1964 Apr;49:649–662. doi: 10.1093/genetics/49.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOLLOWAY B. W. Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol. 1955 Dec;13(3):572–581. doi: 10.1099/00221287-13-3-572. [DOI] [PubMed] [Google Scholar]
  10. HOLLOWAY B. W., JENNINGS P. A. An infectious fertility factor for Pseudomonas aeruginosa. Nature. 1958 Mar 22;181(4612):855–856. doi: 10.1038/181855b0. [DOI] [PubMed] [Google Scholar]
  11. HOLLOWAY B. W., ROLFE B. HOST GENOME CONTROL IN HOST-INDUCED MODIFICATION OF PSEUDOMONAS AERUGINOSA PHAGES. Virology. 1964 Aug;23:595–602. doi: 10.1016/0042-6822(64)90244-2. [DOI] [PubMed] [Google Scholar]
  12. HOLLOWAY B. W. VARIATIONS IN RESTRICTION AND MODIFICATION OF BACTERIOPHAGE FOLLOWING INCREASE OF GROWTH TEMPERATURE OF PSEUDOMONAS AERUGINOSA. Virology. 1965 Apr;25:634–642. doi: 10.1016/0042-6822(65)90091-7. [DOI] [PubMed] [Google Scholar]
  13. Hausmann R., Gomez B., Moody B. Physiological and genetic aspects of abortive infection of a Shigella sonnei strain by coliphage T7. J Virol. 1968 Apr;2(4):335–345. doi: 10.1128/jvi.2.4.335-345.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  15. LEDERBERG S. Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology. 1957 Jun;3(3):496–513. doi: 10.1016/0042-6822(57)90006-5. [DOI] [PubMed] [Google Scholar]
  16. LURIA S. E. Host-induced modifications of viruses. Cold Spring Harb Symp Quant Biol. 1953;18:237–244. doi: 10.1101/sqb.1953.018.01.034. [DOI] [PubMed] [Google Scholar]
  17. Olsen R. H. Isolation and growth of psychrophilic bacteriophage. Appl Microbiol. 1967 Jan;15(1):198–198. doi: 10.1128/am.15.1.198-.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Olsen R. H., Metcalf E. S., Todd J. K. Characteristics of bacteriophages attacking psychrophilic and mesophilic pseudomonads. J Virol. 1968 Apr;2(4):357–364. doi: 10.1128/jvi.2.4.357-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. UETAKE H., TOYAMA S., HAGIWARA S. ON THE MECHANISM OF HOST-INDUCED MODIFICATION. MULTIPLICITY ACTIVATION AND THERMOLABILE FACTOR RESPONSIBLE FOR PHAGE GROWTH RESTRICTION. Virology. 1964 Feb;22:202–213. doi: 10.1016/0042-6822(64)90005-4. [DOI] [PubMed] [Google Scholar]
  20. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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