Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1968 Dec;2(12):1443–1457. doi: 10.1128/jvi.2.12.1443-1457.1968

Electron Microscopic Localization of Herpesvirus-type Particles in Marek's Disease

Mumtaz Ahmed 1, George Schidlovsky 1
PMCID: PMC375489  PMID: 5754505

Abstract

Upon cultivation of chicken lymphoid tumors induced by Georgia isolate of Marek's disease, clusters of refractile rounded cells appeared consistently in the cell cultures. Intranuclear mature, immature, enveloped, and empty herpesvirus-type particles, usually associated with smaller particles, were observed in all rounded cells in the affected areas. Cytoplasmic inclusions containing mature enveloped virus particles were also seen in the rounded cells. Often fibroblasts growing in the vicinity of the cytopathic effect area contained a few herpesvirus-type particles. The original tumors prior to cultivation did not reveal any virus particles, and the virus in infected cultures was always cell-associated. Control cell cultures neither developed the rounded cells nor demonstrated the presence of any type of virus particles.

Full text

PDF
1443

Images in this article

1445Image
on p.1445
1445Image
on p.1445
1446Image
on p.1446
1446Image
on p.1446
1447Image
on p.1447
1447Image
on p.1447
1448Image
on p.1448
1448Image
on p.1448
1449Image
on p.1449
1449Image
on p.1449
1451Image
on p.1451
1451Image
on p.1451
1452Image
on p.1452
1453Image
on p.1453
1454Image
on p.1454
1455Image
on p.1455

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brinkley B. R., Murphy P., Richardson L. C. Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967 Oct;35(1):279–283. doi: 10.1083/jcb.35.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHITWOOD L. A., BRACKEN E. C. REPLICATION OF HERPES SIMPLEX VIRUS IN A METABOLICALLY IMBALANCED SYSTEM. Virology. 1964 Sep;24:116–120. doi: 10.1016/0042-6822(64)90157-6. [DOI] [PubMed] [Google Scholar]
  3. Churchill A. E., Biggs P. M. Agent of Marek's disease in tissue culture. Nature. 1967 Jul 29;215(5100):528–530. doi: 10.1038/215528a0. [DOI] [PubMed] [Google Scholar]
  4. EPSTEIN M. A., ACHONG B. G., BARR Y. M. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Lancet. 1964 Mar 28;1(7335):702–703. doi: 10.1016/s0140-6736(64)91524-7. [DOI] [PubMed] [Google Scholar]
  5. Epstein M. A., Barr Y. M., Achong B. G. Studies with Burkitt's lymphoma. Wistar Inst Symp Monogr. 1965 Sep;4:69–82. [PubMed] [Google Scholar]
  6. FAWCETT D. W. Electron microscope observations on intracellular virus-like particles associated with the cells of the Lucké renal adenocarcinoma. J Biophys Biochem Cytol. 1956 Nov 25;2(6):725–741. doi: 10.1083/jcb.2.6.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henle W. Evidence for viruses in acute leukemia and Burkitt's tumor. Cancer. 1968 Apr;21(4):580–586. doi: 10.1002/1097-0142(196804)21:4<580::aid-cncr2820210406>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lunger P. D., Darlington R. W., Granoff A. Cell-virus relationships in the Lucké renal adenocarcinoma: an ultrastructure study. Ann N Y Acad Sci. 1965 Aug 10;126(1):289–314. doi: 10.1111/j.1749-6632.1965.tb14281.x. [DOI] [PubMed] [Google Scholar]
  10. MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nazerian K., Solomon J. J., Witter R. L., Burmester B. R. Studies on the etiology of Marek's disease. II. Finding of a herpesvirus in cell culture. Proc Soc Exp Biol Med. 1968 Jan;127(1):177–182. doi: 10.3181/00379727-127-32650. [DOI] [PubMed] [Google Scholar]
  12. Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Owen J. J., Moore M. A., Biggs P. M. Chromosome studies in Marek's disease. J Natl Cancer Inst. 1966 Aug;37(2):199–209. [PubMed] [Google Scholar]
  14. RUEBNER B. H., MIYAI K., SLUSSER R. J., WEDEMEYER P., MEDEARIS D. N., Jr MOUSE CYTOMEGALOVIRUS INFECTION. AN ELECTRON MICROSCOPIC STUDY OF HEPATIC PARENCHYMAL CELLS. Am J Pathol. 1964 May;44:799–821. [PMC free article] [PubMed] [Google Scholar]
  15. Rabin E. R., Jenson A. B. Electron microscopic studies of animal viruses with emphasis on in vivo infections. Prog Med Virol. 1967;9:392–450. [PubMed] [Google Scholar]
  16. Rabson A. S., O'Conor G. T., Baron S., Whang J. J., Legallais F. Y. Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child. Int J Cancer. 1966 Jan;1(1):89–106. doi: 10.1002/ijc.2910010112. [DOI] [PubMed] [Google Scholar]
  17. Ruebner B. H., Hirano T., Slusser R., Osborn J., Medearis D. N., Jr Cytomegalovirus infection. Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusions. Am J Pathol. 1966 Jun;48(6):971–989. [PMC free article] [PubMed] [Google Scholar]
  18. SMITH K. O. RELATIONSHIP BETWEEN THE ENVELOPE AND THE INFECTIVITY OF HERPES SIMPLEX VIRUS. Proc Soc Exp Biol Med. 1964 Mar;115:814–816. doi: 10.3181/00379727-115-29045. [DOI] [PubMed] [Google Scholar]
  19. Sevoian M., Chamberlain D. M., Larose R. N. Avian lymphomatosis. V. Air-borne transmission. Avian Dis. 1963 Feb;7(1):102–105. [PubMed] [Google Scholar]
  20. Solomon J. J., Witter R. L., Nazerian K., Burmester B. R. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc Soc Exp Biol Med. 1968 Jan;127(1):173–177. doi: 10.3181/00379727-127-32649. [DOI] [PubMed] [Google Scholar]
  21. Spring S. B., Roizman B., Schwartz J. Herpes simplex virus products in productive and abortive infection. II. Electron microscopic and immunological evidence for failure of virus envelopment as a cause of abortive infection. J Virol. 1968 Apr;2(4):384–392. doi: 10.1128/jvi.2.4.384-392.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vogt P. K. Avian tumor viruses. Adv Virus Res. 1965;11:293–385. doi: 10.1016/s0065-3527(08)60549-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES