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. 1967 Feb;1(1):10–15. doi: 10.1128/jvi.1.1.10-15.1967

Enzyme Induction in Green Monkey Kidney Cultures Infected with Simian Adenovirus

Saul Kit a, Lech J Piekarski a, Del R Dubbs a, Ramon A de Torres a,1, Milton Anken a
PMCID: PMC375499  PMID: 5623953

Abstract

Thymidine kinase was induced after infection of an established strain of green monkey kidney cells (CV-1) with simian adenovirus SV15. Increased levels of thymidine kinase were first observed 8 to 10 hr postinoculation (PI), and the levels increased four- to eightfold by 16 to 24 hr PI. A transient increase (1.5- to 3-fold) of deoxyribonucleic acid (DNA) polymerase activity was also observed about 18 hr PI, but the level of deoxycytidylic deaminase was not enhanced. The inductions of thymidine kinase and DNA polymerase were not obtained when protein synthesis was inhibited with 10−5 M cycloheximide. However, the enzyme increases did take place when infected cultures were treated with 1-β-D-arabinofuranosylcytosine (ara-C), an inhibitor of DNA synthesis and SV15 replication. The incorporation of tritium-labeled thymidine (H3-dT) into DNA was also stimulated 8 to 24 hr after infection with SV15.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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