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. 1967 Feb;1(1):97–109. doi: 10.1128/jvi.1.1.97-109.1967

Ribonucleic Acid Polymerase Catalyzing Synthesis of Double-stranded Arbovirus Ribonucleic Acid

E M Martin 1, J A Sonnabend 1
PMCID: PMC375509  PMID: 5623960

Abstract

The large-particle fraction from the cytoplasm of chick embryo fibroblasts infected with Semliki Forest virus was found to catalyze the incorporation of the 5′-triphosphates of guanosine, adenine, cytidine, and uridine into an acid-insoluble alkali-labile product. The conditions affecting the preparation and assay of this enzyme were investigated. The ribonucleic acid (RNA) polymerase was not present in uninfected cells, and it appeared in infected cells at the time of rapid viral RNA synthesis. The polymerase was found to catalyze the synthesis of a species of RNA which was resistant to ribonuclease and which exhibited the sedimentation properties, buoyant density, and thermal transition temperature of the double-stranded RNA found in vivo in chick cells infected with Semliki forest virus. Attempts to demonstrate that the reaction product of this enzyme also included single-stranded viral RNA were not successful. Although other interpretations are possible, these results give some support to the suggestion that more than one enzyme may be involved in the replication of viral RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASTIER-MANIFACIER S., CORNUET P. ISOLATION OF TURNIP YELLOW MOSAIC VIRUS RNA REPLICASE AND ASYMMETRICAL SYNTHESIS OF POLYNUCLEOTIDES IDENTICAL TO TYMV-RNA. Biochem Biophys Res Commun. 1965 Jan 18;18:283–287. doi: 10.1016/0006-291x(65)90754-0. [DOI] [PubMed] [Google Scholar]
  2. AUGUST J. T., ORTIZ P. J., HURWITZ J. Ribonucleic acid-dependent ribonucleotide incorporation. I. Purification and properties of the enzyme. J Biol Chem. 1962 Dec;237:3786–3793. [PubMed] [Google Scholar]
  3. BALTIMORE D., EGGERS H. J., FRANKLIN R. M., TAMM I. Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production. Proc Natl Acad Sci U S A. 1963 Jun;49:843–849. doi: 10.1073/pnas.49.6.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BALTIMORE D., FRANKLIN R. M. A NEW RIBONUCLEIC ACID POLYMERASE APPEARING AFTER MENGOVIRUS INFECTION OF L-CELLS. J Biol Chem. 1963 Oct;238:3395–3400. [PubMed] [Google Scholar]
  5. BALTIMORE D., FRANKLIN R. M. Preliminary data on a virus-specific enzyme system responsible for the synthesis of viral RNA. Biochem Biophys Res Commun. 1962 Nov 27;9:388–392. doi: 10.1016/0006-291x(62)90021-9. [DOI] [PubMed] [Google Scholar]
  6. BALTIMORE D. IN VITRO SYNTHESIS OF VIRAL RNA BY THE POLIOVIRUS RNA POLYMERASE. Proc Natl Acad Sci U S A. 1964 Mar;51:450–456. doi: 10.1073/pnas.51.3.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bishop J. M., Summers D. F., Levintow L. Characterization of ribonuclease-resistant RNA from poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1273–1281. doi: 10.1073/pnas.54.4.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Borst P., Weissmann C. Replication of viral RNA, 8. Studies on the enzymatic mechanism of replication of MS2 RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):982–987. doi: 10.1073/pnas.54.3.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CANELLAKIS E. S. On the mechanism of incorporation of adenylic acid form adenosine triphosphate into ribonucleic acid by soluble mammalian enzyme systems. Biochim Biophys Acta. 1957 Jul;25(1):217–218. [PubMed] [Google Scholar]
  10. CHENG P. Y. Purification, size, and morphology of a mosquitoborne animal virus, Semliki Forest virus. Virology. 1961 May;14:124–131. doi: 10.1016/0042-6822(61)90139-8. [DOI] [PubMed] [Google Scholar]
  11. DALGARNO L., MARTIN E. M. STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS. Virology. 1965 Jul;26:450–465. doi: 10.1016/0042-6822(65)90008-5. [DOI] [PubMed] [Google Scholar]
  12. DELIUS H., HOFSCHNEIDER P. H. TWO EFFECTS OF INHIBITION OF PROTEIN SYNTHESIS ON THE REPLICATION OF M12 BACTERIOPHAGE RNA. J Mol Biol. 1964 Dec;10:554–556. doi: 10.1016/s0022-2836(64)80078-4. [DOI] [PubMed] [Google Scholar]
  13. Dalgarno L., Martin E. M., Liu S. L., Work T. S. Characterization of the products formed by the RNA polymerases of cells infected with encephalomyocarditis virus. J Mol Biol. 1966 Jan;15(1):77–91. doi: 10.1016/s0022-2836(66)80210-3. [DOI] [PubMed] [Google Scholar]
  14. Edmonds M. A cytidine triphosphate polymerase from thymus nuclei. 1. Purification and properties of the enzyme and its polynucleotide primer. J Biol Chem. 1965 Dec;240(12):4621–4628. [PubMed] [Google Scholar]
  15. Friedman R. M. Interferon production and protein synthesis in chick cells. J Bacteriol. 1966 Mar;91(3):1224–1229. doi: 10.1128/jb.91.3.1224-1229.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Horton E., Liu S. L., Martin E. M., Work T. S. Properties of a virus-induced RNA polymerase in ascites cells infected with encephalomyocarditis virus. J Mol Biol. 1966 Jan;15(1):62–76. doi: 10.1016/s0022-2836(66)80209-7. [DOI] [PubMed] [Google Scholar]
  18. KATZ S., COMB D. G. A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID. J Biol Chem. 1963 Sep;238:3065–3067. [PubMed] [Google Scholar]
  19. KLEMPERER H. G. Manganous ion-dependent incorporation of adenosine-5'-phosphate into ribonucleic acid by an enzyme from rat liver. Biochim Biophys Acta. 1963 Jul 30;72:416–420. [PubMed] [Google Scholar]
  20. Lodish H. F., Zinder N. D. Replication of the RNA of Bacteriophage f2. Science. 1966 Apr 15;152(3720):372–377. doi: 10.1126/science.152.3720.372. [DOI] [PubMed] [Google Scholar]
  21. MONTAGNIER L., SANDERS F. K. REPLICATIVE FORM OF ENCEPHALOMYOCARDITIS VIRUS RIBONUCLEIC ACID. Nature. 1963 Aug 17;199:664–667. doi: 10.1038/199664a0. [DOI] [PubMed] [Google Scholar]
  22. PFEFFERKORN E. R., CLIFFORD R. L. THE ORIGIN OF THE PROTEIN OF SINDBIS VIRUS. Virology. 1964 Jun;23:217–223. doi: 10.1016/0042-6822(64)90285-5. [DOI] [PubMed] [Google Scholar]
  23. PFEFFERKORN E. R., HUNTER H. S. THE SOURCE OF THE RIBONUCLEIC ACID AND PHOSPHOLIPID OF SINDBIS VIRUS. Virology. 1963 Jul;20:446–456. doi: 10.1016/0042-6822(63)90093-x. [DOI] [PubMed] [Google Scholar]
  24. Plagemann P. G., Swim H. E. Symposium on replication of viral nucleic acids. 3. Replication of mengovirus ribonucleic acid. Bacteriol Rev. 1966 Jun;30(2):288–308. doi: 10.1128/br.30.2.288-308.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. TAYLOR J. STUDIES ON THE MECHANISM OF ACTION OF INTERFERON. I. INTERFERON ACTION AND RNA SYNTHESIS IN CHICK EMBRYO FIBROBLASTS INFECTED WITH SEMLIKI FOREST VIRUS. Virology. 1965 Mar;25:340–349. doi: 10.1016/0042-6822(65)90053-x. [DOI] [PubMed] [Google Scholar]
  26. WEISSMANN C., BORST P., BURDON R. H., BILLETER M. A., OCHOA S. REPLICATION OF VIRAL RNA. IV. PROPERTIES OF RNA SYNTHETASE AND ENZYMATIC SYNTHESIS OF MS2 PHAGE RNA. Proc Natl Acad Sci U S A. 1964 May;51:890–897. doi: 10.1073/pnas.51.5.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wykes J. R., Smellie R. M. The synthesis of polyribonucleotides by cytoplasmic enzymes. Biochem J. 1966 May;99(2):347–355. doi: 10.1042/bj0990347. [DOI] [PMC free article] [PubMed] [Google Scholar]

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