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. 1968 Jan;2(1):7–14. doi: 10.1128/jvi.2.1.7-14.1968

Characterization of the Tumorlike (T) Antigen Induced by Type 12 Adenovirus 1

I. Purification of the Antigen from Infected KB Cells and a Hamster Tumor Cell Line

Z Gilead 1,2, H S Ginsberg 1
PMCID: PMC375571  PMID: 5742029

Abstract

The T antigen induced by type 12 adenovirus was purified from KB cells infected in the presence of 10−6m 5-fluoro-2-deoxyuridine to inhibit synthesis of viral capsid antigens. The antigen was purified approximately 200-fold, and the purified product contained only negligible amounts of host-cell contaminants, as judged by the residual radioactivity from 14C-labeled uninfected cells which had been added to infected cells at the initiation of the purification. Immunoelectrophoresis indicated that the purified T-antigen preparation contained a single antigenic species. The T antigen from a hamster cell line (HT-1) derived from a type 12 adenovirus-induced tumor was purified by the same procedure. The T antigens from the two different sources were shown to be immunologically similar by use of a rabbit antiserum prepared against the purified T antigen from infected KB cells and sera from hamsters bearing tumors induced by type 12 adenovirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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