Abstract
After incubation at 37 C for 2 or more days, Basal Medium Eagle (BME) supplemented with 25% fetal calf serum (FCS) was not able to support adequate growth of EB3 and other lines of Burkitt tumor cells. The medium did, however, increase, by a factor of about 10, the number of cells synthesizing herpes-type virus with which the cultures were persistently infected. Not every lot of FCS produced these effects, nor were these effects seen when BME and FCS were incubated separately for 7 days before the medium was completed. At 37 C, appropriate lots of FCS interacted with several of the amino acids present in BME; this interaction resulted in an inhibition of cellular growth, whereas interaction with arginine yielded the virus-enhancing effect. Arginine-free BME, supplemented with 25% FCS and used without prior incubation, prevented cellular replication and promoted viral infection to a similar extent as did preincubated complete medium. Replenishment of arginine reduced, but did not regularly abolish, the virus-enhancing activity of preincubated media. RPMI-1629 medium was less affected by preincubation with FCS because it contained twice the amount of arginine that BME contained. The FCS factors which act upon arginine and other amino acids are not dialyzable and are partially resistant to heating at 56 or 60 C for 30 to 60 min. Calf and horse sera appear to be devoid of these activities. The nature of these interactions, as well as the mechanism by which arginine deficiency enhanced the viral infection, remains to be ascertained.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- EAGLE H., OYAMA V. I., LEVY M., FREEMAN A. E. Myo-Inositol as an essential growth factor for normal and malignant human cells in tissue culture. J Biol Chem. 1957 May;226(1):191–205. [PubMed] [Google Scholar]
- Epstein M. A., Barr Y. M., Achong B. G. Studies with Burkitt's lymphoma. Wistar Inst Symp Monogr. 1965 Sep;4:69–82. [PubMed] [Google Scholar]
- Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henle G., Henle W. Studies on cell lines derived from Burkitt's lymphoma. Trans N Y Acad Sci. 1966 Nov;29(1):71–79. doi: 10.1111/j.2164-0947.1966.tb02253.x. [DOI] [PubMed] [Google Scholar]
- Henle W., Diehl V., Kohn G., Zur Hausen H., Henle G. Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells. Science. 1967 Sep 1;157(3792):1064–1065. doi: 10.1126/science.157.3792.1064. [DOI] [PubMed] [Google Scholar]
- Henle W., Hummeler K., Henle G. Antibody coating and agglutination of virus particles separated from the EB3 line of Burkitt lymphoma cells. J Bacteriol. 1966 Jul;92(1):269–271. doi: 10.1128/jb.92.1.269-271.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hinuma Y., Konn M., Yamaguchi J., Wudarski D. J., Blakeslee J. R., Jr, Grace J. T., Jr Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J Virol. 1967 Oct;1(5):1045–1051. doi: 10.1128/jvi.1.5.1045-1051.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hummeler K., Henle G., Henle W. Fine structure of a virus in cultured lymphoblasts from Burkitt lymphoma. J Bacteriol. 1966 Mar;91(3):1366–1368. doi: 10.1128/jb.91.3.1366-1368.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levy J. A., Henle G. Indirect immunofluorescence tests with sera from African children and cultured Burkitt lymphoma cells. J Bacteriol. 1966 Jul;92(1):275–276. doi: 10.1128/jb.92.1.275-276.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore G. E., Gerner R. E., Franklin H. A. Culture of normal human leukocytes. JAMA. 1967 Feb 20;199(8):519–524. [PubMed] [Google Scholar]
- Moore G. E., Grace J. T., Jr, Citron P., Gerner R., Burns A. Leukocyte cultures of patients with leukemia and lymphomas. N Y State J Med. 1966 Nov 1;66(21):2757–2764. [PubMed] [Google Scholar]
- O'Connor G. T., Rabson A. S. Herpes-like particles in an American lymphoma: preliminary note. J Natl Cancer Inst. 1965 Nov;35(5):899–903. [PubMed] [Google Scholar]
- PELMONT J., MORGAN H. R. Facteurs nutritifs influençant la croissance du virus de l'herpès dans la souche de cellules L de Earle. Ann Inst Pasteur (Paris) 1959 Apr;96(4):448–454. [PubMed] [Google Scholar]
- Rabson A. S., O'Conor G. T., Baron S., Whang J. J., Legallais F. Y. Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child. Int J Cancer. 1966 Jan;1(1):89–106. doi: 10.1002/ijc.2910010112. [DOI] [PubMed] [Google Scholar]
- STEWART S. E., LOVELACE E., WHANG J. J., NGU V. A. BURKITT TUMOR: TISSUE CULTURE, CYTOGENETIC AND VIRUS STUDIES. J Natl Cancer Inst. 1965 Feb;34:319–327. doi: 10.1093/jnci/34.2.319. [DOI] [PubMed] [Google Scholar]
- TANKERSLEY R. W., Jr AMINO ACID REQUIREMENTS OF HERPES SIMPLEX VIRUS IN HUMAN CELLS. J Bacteriol. 1964 Mar;87:609–613. doi: 10.1128/jb.87.3.609-613.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toplin I., Schidlovsky G. Partial purification and electron microscopy of virus in the EB-3 cell line derived from a Burkitt lymphoma. Science. 1966 May 20;152(3725):1084–1085. doi: 10.1126/science.152.3725.1084. [DOI] [PubMed] [Google Scholar]
- Zeve V. H., Lucas L. S., Manaker R. A. Continuous cell culture from a patient with chronic myelogenous leukemia. II. Detection of a herpes-like virus by electron microscopy. J Natl Cancer Inst. 1966 Dec;37(6):761–773. [PubMed] [Google Scholar]