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. 1968 Mar;2(3):248–255. doi: 10.1128/jvi.2.3.248-255.1968

Isolation and Properties of Newcastle Disease Virus Nucleocapsid

D W Kingsbury 1, R W Darlington 1
PMCID: PMC375607  PMID: 5742044

Abstract

Deoxycholate (DOC) disrupted virions of Newcastle disease virus (NDV), releasing viral nucleocapsids. The nucleocapsids sedimented at about 200S in sucrose gradients and measured from 1.3 to 1.4 μ long by electron microscopy. NDV nucleo-capsids were resistant to pancreatic ribonuclease. These nucleocapsids contained all the 50S ribonucleic acid (RNA) in NDV virions, while virus-associated RNA sedimenting at less than 50S was external to the virions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Compans R. W., Choppin P. W. Isolation and properties of the helical nucleocapsid of the parainfluenza virus SV5. Proc Natl Acad Sci U S A. 1967 Apr;57(4):949–956. doi: 10.1073/pnas.57.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Compans R. W., Choppin P. W. The length of the helical nucleocapsid of Newcastle disease virus. Virology. 1967 Oct;33(2):344–346. doi: 10.1016/0042-6822(67)90153-5. [DOI] [PubMed] [Google Scholar]
  3. Duesberg P. H., Robinson W. S. Isolation of the nucleic acid of Newcastle disease virus (NDV). Proc Natl Acad Sci U S A. 1965 Sep;54(3):794–800. doi: 10.1073/pnas.54.3.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hosaka Y., Kitano H., Ikeguchi S. Studies on the pleomorphism of HVJ virons. Virology. 1966 Jun;29(2):205–221. doi: 10.1016/0042-6822(66)90027-4. [DOI] [PubMed] [Google Scholar]
  5. Kingsbury D. W. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. doi: 10.1016/s0022-2836(66)80085-2. [DOI] [PubMed] [Google Scholar]
  6. Kingsbury D. W. Newcastle disease virus complementary RNA: its relationship to the viral genome and its accumulation in the presence or absence of actinomycin D. Virology. 1967 Oct;33(2):227–233. doi: 10.1016/0042-6822(67)90141-9. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  9. SCHAEFER W., ROTT R. [Sub-units of Newcastle disease- and mumps-virus]. Z Naturforsch B. 1959 Oct;14B:629–633. [PubMed] [Google Scholar]
  10. SCHAFER W. Structure of some animal viruses and significance of their components. Bacteriol Rev. 1963 Mar;27:1–17. doi: 10.1128/br.27.1.1-17.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sokol F., Skacianska E., Pivec L. Some properties of Newcastle disease virus ribonucleic acid. Acta Virol. 1966 Jul;10(4):291–300. [PubMed] [Google Scholar]
  12. WATERSON A. P. Two kinds of myxovirus. Nature. 1962 Mar 24;193:1163–1164. doi: 10.1038/1931163a0. [DOI] [PubMed] [Google Scholar]

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