Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1968 Mar;2(3):256–264. doi: 10.1128/jvi.2.3.256-264.1968

Primary Adsorption Site of Phage PBS1: the Flagellum of Bacillus subtilis

Linda M Raimondo 1, Nancy P Lundh 1, Rafael J Martinez 1
PMCID: PMC375608  PMID: 4986906

Abstract

The adsorption of Bacillus subtilis phage PBS1 was studied, and it was demonstrated that the primary adsorption site for this phage is the flagellum of B. subtilis. The capacity of flagella to function for motility may be lost without the loss of their capacity to adsorb the phage and permit infection. Deoxyribonucleic acid injection by the phage is inhibited by cyanide, suggesting the requirement for cellular energy in the infection process.

Full text

PDF
256

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L., NOSSAL G. J., PYE J., ABBOT A. ANTIGENS IN IMMUNITY. I. PREPARATION AND PROPERTIES OF FLAGELLAR ANTIGENS FROM SALMONELLA ADELAIDE. Aust J Exp Biol Med Sci. 1964 Jun;42:267–282. [PubMed] [Google Scholar]
  2. BRENNER S., STENT G. S. Bacteriophage growth in protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1955 Aug;17(4):473–475. doi: 10.1016/0006-3002(55)90409-1. [DOI] [PubMed] [Google Scholar]
  3. Brodetsky A. M., Romig W. R. Characterization of Bacillus subtilis bacteriophages. J Bacteriol. 1965 Dec;90(6):1655–1663. doi: 10.1128/jb.90.6.1655-1663.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  5. Eiserling F. A. The structure of Bacillus subtilis bacteriophage PBS 1. J Ultrastruct Res. 1967 Feb;17(3):342–347. doi: 10.1016/s0022-5320(67)80053-4. [DOI] [PubMed] [Google Scholar]
  6. Frankel R. W., Joys T. M. Adsorption Specificity of Bacteriophage PBS1. J Bacteriol. 1966 Aug;92(2):388–389. doi: 10.1128/jb.92.2.388-389.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grant G. F., Simon M. Use of radioactive antibodies for characterizing antigens and application to the study of flagella synthesis. J Bacteriol. 1968 Jan;95(1):81–86. doi: 10.1128/jb.95.1.81-86.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joys T. M. Correlation between susceptibility to bacteriophage PBS1 and motility in Bacillus subtilis. J Bacteriol. 1965 Dec;90(6):1575–1577. doi: 10.1128/jb.90.6.1575-1577.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lanni Y. T. DNA transfer from phage T5 to host cells: dependence on intercurrent protein synthesis. Proc Natl Acad Sci U S A. 1965 May;53(5):969–973. doi: 10.1073/pnas.53.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MEYNELL E. W. A phage, phi chi, which attacks motile bacteria. J Gen Microbiol. 1961 Jun;25:253–290. doi: 10.1099/00221287-25-2-253. [DOI] [PubMed] [Google Scholar]
  11. ROMIG W. R. Infection of Bacillus subtilis with phenol-extracted bacteriophages. Virology. 1962 Apr;16:452–459. doi: 10.1016/0042-6822(62)90226-x. [DOI] [PubMed] [Google Scholar]
  12. Schade S. Z., Adler J., Ris H. How bacteriophage chi attacks motile bacteria. J Virol. 1967 Jun;1(3):599–609. doi: 10.1128/jvi.1.3.599-609.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schade S., Adler J. Purification and chemistry of bacteriophage chi. J Virol. 1967 Jun;1(3):591–598. doi: 10.1128/jvi.1.3.591-598.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. 1963 May;31:211–217. doi: 10.1099/00221287-31-2-211. [DOI] [PubMed] [Google Scholar]
  16. WEIBULL C. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J Bacteriol. 1953 Dec;66(6):688–695. doi: 10.1128/jb.66.6.688-695.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES