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. 1968 Apr;2(4):320–326. doi: 10.1128/jvi.2.4.320-326.1968

Host-controlled Restriction of T-even Bacteriophages: Relation of Four Bacterial Deoxyribonucleases to Restriction

Joseph Eigner 1,1, Stephen Block 1,2
PMCID: PMC375617  PMID: 4911846

Abstract

Escherichia coli strains B and K-12, which restrict growth of nonglucosylated T- even phage (T* phage), and nonrestricting strains (Shigella sonnei and mutants of E. coli B) were tested for levels of endonuclease I and exonucleases I, II, and III, by means of in vitro assyas. Cell-free extracts freed from deoxyribonucleic acid (DNA) were examined with three substrates: E. coli DNA, T*2 DNA, and T2 DNA. Both restricting and nonrestricting strains had comparable levels of the four nuclease activities and had similar patterns of preference for the three substrates. In addition, mutants of E. coli B and K-12 that lack endonuclease I were as effective as their respective wild types in restricting T* phage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cummings D. J., Mondale L. Density-gradient banding of denatured deoxyribonucleic acid in cesium sulphate. Biochim Biophys Acta. 1966 Jul 13;120(3):448–453. doi: 10.1016/0926-6585(66)90311-6. [DOI] [PubMed] [Google Scholar]
  3. Eigner J., Doty P. The native, denatured and renatured states of deoxyribonucleic acid. J Mol Biol. 1965 Jul;12(3):549–580. doi: 10.1016/s0022-2836(65)80312-6. [DOI] [PubMed] [Google Scholar]
  4. HATTMAN S. THE FUNCTIONING OF T-EVEN PHAGES WITH UNGLUCOSYLATED DNA IN RESTRICTING ESCHERICHIA COLI HOST CELLS. Virology. 1964 Nov;24:333–348. doi: 10.1016/0042-6822(64)90171-0. [DOI] [PubMed] [Google Scholar]
  5. LEHMAN I. R., RICHARDSON C. C. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. IV. AN EXONUCLEASE ACTIVITY PRESENT IN PURIFIED PREPARATIONS OF DEOXYRIBONUCLEIC ACID POLYMERASE. J Biol Chem. 1964 Jan;239:233–241. [PubMed] [Google Scholar]
  6. LEHMAN I. R., ROUSSOS G. G., PRATT E. A. The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease. J Biol Chem. 1962 Mar;237:819–828. [PubMed] [Google Scholar]
  7. LEHMAN I. R. The deoxyribonucleases of Escherichia coli. I. Purification and properties of a phosphodiesterase. J Biol Chem. 1960 May;235:1479–1487. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Massie H. R., Zimm B. H. THE USE OF HOT PHENOL IN PREPARING DNA. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1641–1643. doi: 10.1073/pnas.54.6.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Molholt B., Fraser D. Host-controlled restriction of T-even bacteriophages: relation of endonuclease I and T-even-induced nucleases to restriction. J Virol. 1968 Apr;2(4):313–319. doi: 10.1128/jvi.2.4.313-319.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  12. RICHARDSON C. C., LEHMAN I. R., KORNBERG A. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY. J Biol Chem. 1964 Jan;239:251–258. [PubMed] [Google Scholar]
  13. Revel H. R. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12. Virology. 1967 Apr;31(4):688–701. doi: 10.1016/0042-6822(67)90197-3. [DOI] [PubMed] [Google Scholar]
  14. Richardson C. C. Influence of glucosylation of deoxyribonucleic acid on hydrolysis by deoxyribonucleases of Escherichia coli. J Biol Chem. 1966 May 10;241(9):2084–2092. [PubMed] [Google Scholar]
  15. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  16. SHORTMAN K., LEHMAN I. R. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. VI. CHANGES IN ENZYME LEVELS IN RESPONSE TO ALTERATIONS IN PHYSIOLOGICAL STATE. J Biol Chem. 1964 Sep;239:2964–2974. [PubMed] [Google Scholar]
  17. Takano T., Watanabe T., Fukasawa T. Specific inactivation of infectious lambda DNA by sonicates of restrictive bacteria with R factors. Biochem Biophys Res Commun. 1966 Oct 20;25(2):192–198. doi: 10.1016/0006-291x(66)90579-1. [DOI] [PubMed] [Google Scholar]

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