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Journal of Virology logoLink to Journal of Virology
. 1968 Apr;2(4):357–364. doi: 10.1128/jvi.2.4.357-364.1968

Characteristics of Bacteriophages Attacking Psychrophilic and Mesophilic Pseudomonads 1

R H Olsen 1, Eleanor S Metcalf 1, James K Todd 1,2
PMCID: PMC375621  PMID: 4986902

Abstract

Ten Pseudomonas phage were isolated by sewage enrichment. Five psychrophilic and five mesophilic phage were selected for a description of some of their biological properties. In addition to growth on psychrophilic hosts, the psychrophilic phage studied were also able to grow on a mesophilic host within its growth temperature range. Latent periods for psychrophilic phage at 3.5 C were 6 to 12 hr and at 25 C were 30 to 60 min. Mesophilic phage had a latent period of 85 to 190 min at 25 C and 35 to 85 min at 37 C. Psychrophilic phage were significantly more heat-sensitive than the mesophilic phage. Of all the parameters studied, only thermal sensitivity correlated with growth at 3.5 C. Phage used in this study had a deoxyribonucleic acid base composition ranging from 39.6 to 68.2% guanine plus cytosine, deduced from melting temperature measurements.

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Selected References

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