Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1968 Apr;2(4):376–383. doi: 10.1128/jvi.2.4.376-383.1968

Adenovirus Transformation of Hamster Embryo Cells

Bruce C Casto 1
PMCID: PMC375623  PMID: 4316017

Abstract

Inoculation of hamster embryo cell cultures with human adenovirus type 12 (Ad12) or simian adenovirus (SA7) resulted in the formation of foci of morphologically transformed cells within 12 days. The rapid appearance of well-defined foci was dependent upon the transfer of cells into new plates, with sufficient dilution after virus adsorption, and was independent of virus dose. Dose-response studies showed linearity of focus formation with dilution of Ad12 or SA7. Results averaged from several experiments show plaque-forming unit to focus-forming unit ratios of approximately 1.8 × 106 for Ad12 and 2.6 × 105 for SA7. Other experiments showed that most of the adenovirus involved in transformation was adsorbed by 3 hr. Cell lines derived from SA7 transformed cells produced tumors within 19 days when inoculated intradermally into young adult hamsters. Such cell-induced tumors histologically resembled SA7 virus-induced hamster tumors. Formation of tumors with SA7 transformed cells was inhibited by prior immunization of test animals with SA7 or Ad12 virus.

Full text

PDF
376

Images in this article

377Image
on p.377
378Image
on p.378
379Image
on p.379

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black P. H. Transformation of mouse cell line 3T3 by SV40: dose response relationship and correlation with SV40 tumor antigen production. Virology. 1966 Apr;28(4):760–763. doi: 10.1016/0042-6822(66)90262-5. [DOI] [PubMed] [Google Scholar]
  2. Freeman A. E., Black P. H., Wolford R., Huebner R. J. Adenovirus type 12-rat embryo transformation system. J Virol. 1967 Apr;1(2):362–367. doi: 10.1128/jvi.1.2.362-367.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kusano T., Yamane I. Transformation in vitro of the embryonal hamster brain cells by human adenovirus type 12. Tohoku J Exp Med. 1967 Jun;92(2):141–150. doi: 10.1620/tjem.92.141. [DOI] [PubMed] [Google Scholar]
  4. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  5. MCBRIDE W. D., WIENER A. IN VITRO TRANSFORMATION OF HAMSTER KIDNEY CELLS BY HUMAN ADENOVIRUS TYPE 12. Proc Soc Exp Biol Med. 1964 Apr;115:870–874. doi: 10.3181/00379727-115-29060. [DOI] [PubMed] [Google Scholar]
  6. McAllister R. M., Goodheart C. R., Mirabal V. Q., Huebner R. J. Human adenoviruses: tumor production in hamsters by type 12 and 18 grown from single plaques. Proc Soc Exp Biol Med. 1966 Jun;122(2):455–458. doi: 10.3181/00379727-122-31160. [DOI] [PubMed] [Google Scholar]
  7. McAllister R. M., Macpherson I. Transformation of a hamster cell line by adenovirus type 12. J Gen Virol. 1968 Jan;2(1):99–106. doi: 10.1099/0022-1317-2-1-99. [DOI] [PubMed] [Google Scholar]
  8. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rafajko R. R. Routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12. J Natl Cancer Inst. 1967 Apr;38(4):581–588. [PubMed] [Google Scholar]
  10. STOKER M., ABEL P. Conditions affecting transformation by polyoma virus. Cold Spring Harb Symp Quant Biol. 1962;27:375–386. doi: 10.1101/sqb.1962.027.001.035. [DOI] [PubMed] [Google Scholar]
  11. Strohl W. A., Rabson A. S., Rouse H. Adenovirus tumorigenesis: role of the viral genome in determining tumor morphology. Science. 1967 Jun 23;156(3782):1631–1633. doi: 10.1126/science.156.3782.1631. [DOI] [PubMed] [Google Scholar]
  12. Todaro G. J., Green H. Cell growth and the initiation of transformation by SV40. Proc Natl Acad Sci U S A. 1966 Feb;55(2):302–308. doi: 10.1073/pnas.55.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Todaro G. J., Green H. High frequency of SV40 transformation of mouse cell line 3T3. Virology. 1966 Apr;28(4):756–759. doi: 10.1016/0042-6822(66)90261-3. [DOI] [PubMed] [Google Scholar]
  14. WILLIAMS J. F., TILL J. E. TRANSFORMATION OF RAT EMBRYO CELLS IN CULTURE BY POLYOMA VIRUS. Virology. 1964 Nov;24:505–508. doi: 10.1016/0042-6822(64)90197-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES