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. 1968 May;2(5):415–420. doi: 10.1128/jvi.2.5.415-420.1968

Rescue of Temperature-sensitive Poliovirus 1

F Richard Nichol 1,2, Daniel R Tershak 1
PMCID: PMC375629  PMID: 4301311

Abstract

A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the mutant yield obtained under permissive conditions. Rescued virus underwent replication, because the progeny of 32P-labeled LSc were not radiosensitive. Serum inactivation studies with Mahoney specific antiserum indicated that a small amount of phenotypic mixing occurred among the rescued particles. The temperature-sensitive event occurred between 2 and 4 hr postinfection in the developmental cycle of LSc. Neither viral polymerase activity nor virus-induced ribonucleic acid could be demonstrated in infected cells between 2 and 4 hr after infection at 40 C with the temperature-sensitive mutant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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