Abstract
One hour after infection of chick fibroblasts with Semliki Forest virus (SFV), a viral ribonucleic acid (RNA) structure is present which has many of the properties described for the replicative intermediate of several RNA bacteriophages. These properties include a polydisperse nature on sucrose density gradient analysis, ribonuclease resistance, a variation in sedimentation pattern associated with changes in salt concentration, and recovery of infectious viral RNA upon denaturation. Most of the replicative intermediate present in SFV infection appears to be membrane-associated.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltimore D., Girard M. An intermediate in the synthesis of poliovirus RNA. Proc Natl Acad Sci U S A. 1966 Aug;56(2):741–748. doi: 10.1073/pnas.56.2.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borst P., Weissmann C. Replication of viral RNA, 8. Studies on the enzymatic mechanism of replication of MS2 RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):982–987. doi: 10.1073/pnas.54.3.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deusberg P. H., Robinson W. S. On the structure and replication of influenza virus. J Mol Biol. 1967 May 14;25(3):383–405. doi: 10.1016/0022-2836(67)90193-3. [DOI] [PubMed] [Google Scholar]
- FENWICK M. L., ERIKSON R. L., FRANKLIN R. M. REPLICATION OF THE RNA OF BACTERIOPHAGE R17. Science. 1964 Oct 23;146(3643):527–530. doi: 10.1126/science.146.3643.527. [DOI] [PubMed] [Google Scholar]
- Francke B., Hofschneider P. H. Infectious nucleic acids of e. Coli bacteriophages, ix. Sedimentation constants and strand integrity of infectious m12 phage replicative-form RNA. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1883–1890. doi: 10.1073/pnas.56.6.1883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedman R. M., Berezesky I. K. Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J Virol. 1967 Apr;1(2):374–383. doi: 10.1128/jvi.1.2.374-383.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedman R. M. Protein synthesis directed by an arbovirus. J Virol. 1968 Jan;2(1):26–32. doi: 10.1128/jvi.2.1.26-32.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLY R. B., SINSHEIMER R. L. A NEW RNA COMPONENT IN MS2-INFECTED CELLS. J Mol Biol. 1964 Apr;8:602–605. doi: 10.1016/s0022-2836(64)80015-2. [DOI] [PubMed] [Google Scholar]
- Katz L., Penman S. The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells. Biochem Biophys Res Commun. 1966 May 25;23(4):557–560. doi: 10.1016/0006-291x(66)90765-0. [DOI] [PubMed] [Google Scholar]
- Martin E. M., Sonnabend J. A. Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J Virol. 1967 Feb;1(1):97–109. doi: 10.1128/jvi.1.1.97-109.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mécs E., Sonnabend J. A., Martin E. M., Fantes K. H. The effect of interferon on the synthesis of RNA in chick cells infected with Semliki forest virus. J Gen Virol. 1967 Jan;1(1):25–40. doi: 10.1099/0022-1317-1-1-25. [DOI] [PubMed] [Google Scholar]
- RICHTER A., WECKER E. The reaction of EEE virus preparations with sodium deoxycholate. Virology. 1963 Jun;20:263–268. doi: 10.1016/0042-6822(63)90114-4. [DOI] [PubMed] [Google Scholar]
- SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
- SEBRING E. D., SALZMAN N. P. AN IMPROVED PROCEDURE FOR MEASURING THE DISTRIBUTION OF P32O4--AMONG THE NUCLEOTIDES OF RIBONUCLEIC ACID. Anal Biochem. 1964 May;8:126–129. doi: 10.1016/0003-2697(64)90177-0. [DOI] [PubMed] [Google Scholar]
- Sonnabend J. A., Martin E. M., Mécs E. Viral specific RNAs in infected cells. Nature. 1967 Jan 28;213(5074):365–367. doi: 10.1038/213365a0. [DOI] [PubMed] [Google Scholar]