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. 1968 Jun;2(6):610–620. doi: 10.1128/jvi.2.6.610-620.1968

Disruption of T-even Bacteriophages by Dimethyl Sulfoxide

Donald J Cummings 1, V A Chapman 1, S S DeLong 1
PMCID: PMC375655  PMID: 5697471

Abstract

Dimethyl sulfoxide (DMSO) disrupted T-even bacteriophages as well as λ bacteriophage. The component substructures of T2L, T4B01, or T6, in particular heads, were readily isolated after treatment with 67% DMSO (v/v). In contrast, concentrations of DMSO above 50% not only separated heads from tails of bacteriophage λ but led to degradation of the λ heads. Examination of the isolated free heads of T-even bacteriophage indicated that a distinct neck substructure was attached to one apex of the head. On some free tails a similar neck substructure was also found at the proximal end of the sheath. The dimensions of this neck substructure were found to be about 130 by 180 A; by virtue of its size and morphological attachment to the free heads, it was concluded that this was a distinct substructure and not an extension of the tail tube.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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