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. 1968 Sep;2(9):869–877. doi: 10.1128/jvi.2.9.869-877.1968

Reovirus-induced Ribonucleic Acid Polymerase

Y Watanabe 1, C J Gauntt 1,1, A F Graham 1
PMCID: PMC375706  PMID: 5725319

Abstract

A virus-induced ribonucleic acid (RNA) polymerase activity was found in L cells infected with type 3 reovirus. Most of the enzyme is associated with the “large particle” fraction of the infected cells. The enzyme first appeared at 3 to 5 hr after infection and increased in amount until 7 to 9 hr. All four ribonucleoside triphosphates are incorporated in vitro into an acid-insoluble form by the enzyme. The major part of the product formed in vitro is a double-stranded RNA indistinguishable from viral RNA by electrophoresis on polyacrylamide gel. Approximately 40% of the product is a single-stranded RNA of relatively small molecular weight. More than 95% of the nucleotides incorporated into double-stranded RNA by the enzyme are bound in internal 3′-5′-phosphodiester linkages extending back from both 3′- and 5′-termini of the RNA strands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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