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. 1968 Sep;2(9):937–943. doi: 10.1128/jvi.2.9.937-943.1968

Use of Zonal Ultracentrifuge Systems for Biophysical Studies of Rhinoviruses

John L Gerin 1,1, Ward R Richter 1,2, James D Fenters 1,3, Jacob C Holper 1
PMCID: PMC375715  PMID: 4302190

Abstract

This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinovirus infectivity and antigenicity. Additional purification was achieved by the combined use of continuous-flow centrifugation and isopycnic banding procedures. Two particle sizes were found to be associated with the virus-infected cell harvests. The infectious 22-nm particle banded in density ranges of 1.38 to 1.40 g/cm3 in CsCl and 1.26 to 1.27 g/cm3 in potassium citrate. The 8.0 nm capsomere was composed of 2.0 nm subunits and banded with a density of protein at 1.28 g/cm3 in CsCl. Equivalent sedimentation coefficients of 155 or 185, depending on particle density in sucrose, were calculated from rate zonal experiments by use of the B-IV zonal rotor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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