Abstract
Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens V-1 is temperature-sensitive. At 25 C and 30 C, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 C, although bacterial growth was not inhibited, phage release was suppressed. Phage synthesis was induced by heat shock, 42 C for 30 min, ultraviolet irradiation, and mitomycin C. Induction by ultraviolet light was unusual—an immediate rise in phage titer followed irradiation. A large increase occurred after a 90-min latent period. The lysogenic strain was cured of the phage by incubation at 37 C, and the cured strain produced plant tumors.
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Selected References
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- BEARDSLEY R. E. Lysogenicity in Agrobacterium tumefaciens. J Bacteriol. 1960 Aug;80:180–187. doi: 10.1128/jb.80.2.180-187.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heberlein G. T., Lippincott J. A. Ultraviolet-induced changes in the infectivity of Agrobacterium tumefaciens. J Bacteriol. 1967 Apr;93(4):1246–1253. doi: 10.1128/jb.93.4.1246-1253.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iglewski W. J., Franklin R. M. Denaturation and renaturation of viral RNA. 3. Purification of the complementary strand of R17 RNA. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1019–1024. doi: 10.1073/pnas.58.3.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE M. Effect of mitomycin C on interactions between temperate phages and bacteria. Virology. 1961 Apr;13:493–499. doi: 10.1016/0042-6822(61)90280-x. [DOI] [PubMed] [Google Scholar]
- LIPPINCOTT J. A., LIPPINCOTT B. B. AGROBACTERIUM TUMEFACIENS: THERMAL INACTIVATION OF TUMOR-INDUCING ABILITY. Science. 1965 Mar 26;147(3665):1578–1579. doi: 10.1126/science.147.3665.1578. [DOI] [PubMed] [Google Scholar]
- Lippincott J. A., Heberlein G. T. The quantitative determination of the infectivity of Agrobacterium tumefaciens. Am J Bot. 1965 Sep;52(8):856–863. [PubMed] [Google Scholar]
- Stonier T., McSharry J., Speitel T. Agrobacterium tumefaciens Conn. IV. Bacteriophage PB21 and its inhibitory effect on tumor induction. J Virol. 1967 Apr;1(2):268–273. doi: 10.1128/jvi.1.2.268-273.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmerer R. P., Hamilton R. H., Pootjes C. Isolation and morphology of temperature Agrobacterium tumefaciens bacteriophage. J Bacteriol. 1966 Sep;92(3):746–750. doi: 10.1128/jb.92.3.746-750.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]