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. 1969 Feb;3(2):187–197. doi: 10.1128/jvi.3.2.187-197.1969

Electron Microscopy of Measles Virus Replication

Masuyo Nakai 1,2,1, David T Imagawa 1,2
PMCID: PMC375751  PMID: 5774139

Abstract

Replication of measles virus in HeLa cells was examined by electron microscopy with ultrathin sectioning and phosphotungstic acid negative staining methods. The cytoplasmic inclusion bodies consisted of masses of helical nucleocapsid which was similar in structure to the nucleocapsid found in measles virions. The cytoplasmic helical nucleocapsid appeared to align near the HeLa cell membrane, and the membrane differentiated into the internal membrane of the viral envelope and the outer layer of the short projections. The viral particles were released by a budding process involving incorporation into the viral envelope of membrane which was contiguous to but morphologically altered from the membrane of the HeLa cells. The intranuclear inclusion bodies were composed of tubular structures similar to those found in the cytoplasmic inclusion bodies. These structures aggregated to crystalline arrangement. The relationship between nuclear inclusion body and replication of measles virus was not clear.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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