Abstract
A rapid, simple assay method has been developed for antiviral antibodies. The technique has been applied to antisera, immune γ-globulins, and immunospecifically purified antibody for two strains of influenza virus, Asian 305 and PR8, and to antisera to tobacco mosaic virus. Turbidity changes due to the specific interaction of a virus with its antibody were measured by the increase in optical density in a sensitive wavelength region, e.g., 436 nm. Successful application of the method required that nonspecific effects which give rise to turbidity changes be eliminated. This was accomplished by proper choice of ionic strength (0.3 m) and pH (5.5), and by the addition of normal serum or serum albumin to the virus before contact with the antibody. Sensitivity of the method allowed quantitation of antibody down to the level of 10 μg of antibody protein per ml. The specificity of the reaction causing the turbidity change was established by experiments which showed that precipitation of virus-antibody complexes removed the reactive component in the serum, and by the absence of turbidity changes for nonspecific pairs (virus plus unrelated antisera).
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Selected References
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- BRADISH C. J., CRAWFORD L. V. Biophysical studies of the interactions between the viruses of tobacco mosaic and tomato bushy stunt and their rabbit antisera. Virology. 1960 May;11:48–78. doi: 10.1016/0042-6822(60)90055-6. [DOI] [PubMed] [Google Scholar]
- Dandliker W. B., Alonso R., de Saussure V. A., Kierszenbaum F., Levison S. A., Schapiro H. C. The effect of chaotropic ions on the dissociation of antigen-antibody complexes. Biochemistry. 1967 May;6(5):1460–1467. doi: 10.1021/bi00857a031. [DOI] [PubMed] [Google Scholar]
- GOLDBERG R. J., CAMPBELL D. H. The light-scattering properties of an antigen-antibody reaction. J Immunol. 1951 Jan;66(1):79–86. [PubMed] [Google Scholar]
- Neurath A. R., Rubin B. A., Fontes A. K. Fluorometric determination of submicrogram amounts of antigens: application of the method to influenza viral antigens. Life Sci. 1966 Dec;5(24):2271–2277. doi: 10.1016/0024-3205(66)90062-2. [DOI] [PubMed] [Google Scholar]
- RAGETLI H. W., WEINTRAUB M. IMMUNO-OSMOPHORESIS, A RAPID AND SENSITIVE METHOD FOR EVALUATING VIRUSES. Science. 1964 May 22;144(3621):1023–1024. doi: 10.1126/science.144.3621.1023. [DOI] [PubMed] [Google Scholar]