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. 1969 Mar;3(3):331–336. doi: 10.1128/jvi.3.3.331-336.1969

Effect of Hydroxyurea on Replication of Bacteriophage T4 in Escherichia coli1

H R Warner 1, M D Hobbs 1
PMCID: PMC375772  PMID: 4889472

Abstract

Hydroxyurea inhibited the replication of bacteriophage T4 in Escherichia coli B. The concentration of hydroxyurea required to inhibit net deoxyribonucleic acid (DNA) synthesis 50% was about 50-fold less than that required in uninfected cells. Even in the presence of high hydroxyurea concentrations, phage DNA was readily synthesized from the products of breakdown of the E. coli DNA, and viable phage were made. Deoxyribonucleotide, but not ribonucleotide, synthesis was strongly inhibited in the presence of hydroxyurea. The data indicate that hydroxyurea specifically inhibits de novo DNA synthesis in E. coli infected with bacteriophage T4 by inhibiting the ribonucleoside diphosphate reductase system, but does not affect DNA synthesis at subsequent steps.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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