Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1969 Apr;3(4):429–438. doi: 10.1128/jvi.3.4.429-438.1969

Ribonucleic Acid Synthesis in Chick Embryo Cells Infected with Fowl-Plague Virus

J J Skehel 1, D C Burke 1
PMCID: PMC375788  PMID: 4977166

Abstract

Increases in ribonucleic acid (RNA) polymerase activity were detected in both the nuclear and ribosomal fractions of chick embryo cells infected with fowl-plague virus. These were observed only in the presence of all four nucleoside triphosphates and were not affected by actinomycin D. The RNA polymerase activity of the ribosomal fraction was shown to be associated with a component of infected cells of sedimentation coefficient approximately 70S. This component also contained infected cell-specific RNA and protein molecules.

Full text

PDF
429

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry R. D., Davies P. The sedimentation of influenza virus and its RNA in sucrose density gradients. J Gen Virol. 1968 Jan;2(1):59–69. doi: 10.1099/0022-1317-2-1-59. [DOI] [PubMed] [Google Scholar]
  2. Borland R., Mahy B. W. Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in cells infected with influenza virus. J Virol. 1968 Jan;2(1):33–39. doi: 10.1128/jvi.2.1.33-39.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deusberg P. H., Robinson W. S. On the structure and replication of influenza virus. J Mol Biol. 1967 May 14;25(3):383–405. doi: 10.1016/0022-2836(67)90193-3. [DOI] [PubMed] [Google Scholar]
  4. Duesberg P. H. The RNA of influenza virus. Proc Natl Acad Sci U S A. 1968 Mar;59(3):930–937. doi: 10.1073/pnas.59.3.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
  6. HENDLER R. W. PROCEDURE FOR SIMULTANEOUS ASSAY OF TWO BETA-EMITTING ISOTOPES WITH THE LIQUID SCINTILLATION COUNTING TECHNIQUE. Anal Biochem. 1964 Jan;7:110–120. doi: 10.1016/0003-2697(64)90125-3. [DOI] [PubMed] [Google Scholar]
  7. Hay A. J., Skehel J. J., Burke D. C. Proteins synthesized in chick cells following infection with Semliki Forest virus. J Gen Virol. 1968 Sep;3(2):175–184. doi: 10.1099/0022-1317-3-2-175. [DOI] [PubMed] [Google Scholar]
  8. Ho P. P., Walters C. P. Influenza virus-induced ribonucleic acid nucleotidyltransferase and the effect of actinomycin D on its formation. Biochemistry. 1966 Jan;5(1):231–235. doi: 10.1021/bi00865a030. [DOI] [PubMed] [Google Scholar]
  9. LINDENMANN J., BURKE D. C., ISAACS A. Studies on the production, mode of action and properties of interferon. Br J Exp Pathol. 1957 Oct;38(5):551–562. [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Nayak D. P., Baluda M. A. Isolation and partial characterization of nucleic acid of influenza virus. J Virol. 1967 Dec;1(6):1217–1223. doi: 10.1128/jvi.1.6.1217-1223.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nayak D. P., Baluda M. A. Ribonucleic acid synthesis in cells infected with influenza virus. J Virol. 1968 Feb;2(2):99–109. doi: 10.1128/jvi.2.2.99-109.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PETERMANN M. L., PAVLOVEC A. Ribonucleoprotein from a rat tumor, the Jensen sarcoma. II. Complexes formed with hemoglobin. J Biol Chem. 1961 Dec;236:3235–3239. [PubMed] [Google Scholar]
  14. Pons M. W., Hirst G. K. Polyacrylamide gel electrophoresis of influenza virus RNA. Virology. 1968 Feb;34(2):385–388. doi: 10.1016/0042-6822(68)90257-2. [DOI] [PubMed] [Google Scholar]
  15. Pons M. W. Studies on influenza virus ribonucleic acid. Virology. 1967 Mar;31(3):523–531. doi: 10.1016/0042-6822(67)90234-6. [DOI] [PubMed] [Google Scholar]
  16. ROTT R., SCHOLTISSEK C. EINFLUSS VON ACTINOMYCIN AUF DIE VERMEHRUNG VON MYXOVIREN. Z Naturforsch B. 1964 Apr;19:316–323. doi: 10.1007/BF01191316. [DOI] [PubMed] [Google Scholar]
  17. Scholtissek C., Becht H., Drzeniek R. Biochemical studies on the cytopathic effect of influenza viruses. J Gen Virol. 1967 Apr;1(2):219–225. doi: 10.1099/0022-1317-1-2-219. [DOI] [PubMed] [Google Scholar]
  18. Silpananta P., Dunstone J. R., Ogston A. G. Fractionation of ahyaluronic acid preparation in a density gradient. Some properties of the hyaluronic acid. Biochem J. 1968 Aug;109(1):43–50. doi: 10.1042/bj1090043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Skehel J. J., Burke D. C. Interferon production by Semliki Forest virus inactivated with hydroxylamine. J Gen Virol. 1968 Jul;3(1):35–42. doi: 10.1099/0022-1317-3-1-35. [DOI] [PubMed] [Google Scholar]
  20. Skehel J. J., Hay A. J., Burke D. C., Cartwright L. N. Effects of actinomycin D and 2-mercapto-1-(beta-4-pyridethyl) benzimidazole on the incorporation of [3H]uridine by chick embryo cells. Biochim Biophys Acta. 1967 Jul 18;142(2):430–439. doi: 10.1016/0005-2787(67)90624-7. [DOI] [PubMed] [Google Scholar]
  21. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Walters S., Burke D. C., Skehel J. J. Interferon production and RNA inhibitors. J Gen Virol. 1967 Jul;1(3):349–362. doi: 10.1099/0022-1317-1-3-349. [DOI] [PubMed] [Google Scholar]
  23. White D. O., Cheyne I. M. Early events in the eclipse phase of influenza and parainfluenza virus infection. Virology. 1966 May;29(1):49–59. doi: 10.1016/0042-6822(66)90195-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES