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. 1969 May;3(5):477–483. doi: 10.1128/jvi.3.5.477-483.1969

Substituted Sialic Acid Prosthetic Groups as Determinants of Viral Hemagglutination

Barbara Levinson 1,1, Duncan Pepper 1,2, George Belyavin 1
PMCID: PMC375797  PMID: 4306720

Abstract

Inhibitors of hemagglutination by type A2 influenza virus and a recently isolated strain of type B influenza virus were separated by sucrose density gradient centrifugation and agarose gel filtration from horse serum. Using selected reagents, it was demonstrated that the active substituent on the horse serum inhibitor of A2 influenza virus was 4-O-acetyl-N-acetylneuraminic acid; however, the active substituent on the inhibitor of the influenza B virus was shown to be N-acetylneuraminic acid (NANA). Sodium metaperiodate treatment of a component of horse serum resulted in a 10 to 15-fold enhancement of inhibitory activity against the type B virus, whereas the A2 inhibitor was completely destroyed. Since this enhancement did not occur with influenza B viruses isolated prior to 1965, it was considered that this sensitivity to an oxidized NANA glycoside may have been a reflection of an antigenic change which occurred at that time. The use of different virus strains and selected chemical reagents to define the important sialic acid prosthetic groups active in inhibition was described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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