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. 1969 Jun;3(6):570–577. doi: 10.1128/jvi.3.6.570-577.1969

Effect of Ultraviolet Irradiation and Actinomycin D on Polyoma Virus Replication in Mouse Embryo Cell Cultures

J M Bowen 1, R G Hughes 1, L Dmochowski 1
PMCID: PMC375814  PMID: 4307903

Abstract

Ultraviolet irradiation and actinomycin D impair the capacity of mouse embryo (ME) cells to support the replication of polyoma virus, but not of encephalomyocarditis (EMC) virus. The loss in capacity for polyoma virus synthesis was an “all-or-none” effect and followed closely upon the loss in cellular capacity for clone formation. Cells treated with either agent produced polyoma “T” antigen, but did not synthesize polyoma structural protein. Infection of untreated ME cells with polyoma virus produced marked stimulation of both deoxyribonucleic acid (DNA) synthesis and ribonucleic acid (RNA) synthesis. ME cell cultures irradiated with ultraviolet for 30 sec at 60 μw/cm2 or treated with actinomycin D at 0.1 μg/ml for 6 hr prior to infection were incapable of synthesizing DNA or RNA, even after infection with polyoma virus. Irradiation of cells during infection produced cessation of synthesis of both RNA and DNA. Addition of actinomycin D during infection did not inhibit DNA synthesis but abolished RNA synthesis and reduced the yield of polyoma virus to 10% of that in untreated infected cultures. Both agents lost the ability to prevent replication of a full yield of polyoma virus when administered 30 hr after infection or later. The period after which neither agent inhibited polyoma replication corresponded with the period at which maximal RNA synthesis in untreated infected cultures had subsided. It can be concluded on the basis of the data presented that the functional integrity of the mouse embryo cell genome is required for the replication of polyoma virus, but not for EMC virus. Whereas the requirement for cellular DNA-dependent RNA synthesis for polyoma virus replication has been demonstrated, the exact nature of the host-cell function remains to be elucidated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERECZKY E., HUGHES R., BOWEN J. M., MUNYON W., DMOCHOWSKI L. STUDY OF DNA SYNTHESIS AND ANTIGEN FORMATION IN POLYOMA VIRUS-INFECTED MOUSE EMBRYO CELLS BY AUTORADIOGRAPHY AND IMMUNOFLUORESCENCE. Tex Rep Biol Med. 1965;23:3–15. [PubMed] [Google Scholar]
  2. BORENFREUND E., KRIM M., BENDICH A. EFFECTS OF MITOMYCIN C ON THE INFECTION OF CELLS BY POLYOMA VIRUS ANND VIRUS ITS DNA. Virology. 1965 Mar;25:393–400. doi: 10.1016/0042-6822(65)90060-7. [DOI] [PubMed] [Google Scholar]
  3. Basilico C., Marin G., di Mayorca G. Requirement for the integrity of the viral genome for the induction of host DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1966 Jul;56(1):208–215. doi: 10.1073/pnas.56.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ben-Porat T., Kaplan A. S., Tennant R. W. Effect of 5-fluorouracil on the multiplication of a virulent virus (pseudorabies) and an oncogenic virus (polyoma). Virology. 1967 Jul;32(3):445–456. doi: 10.1016/0042-6822(67)90296-6. [DOI] [PubMed] [Google Scholar]
  5. Benjamin T. L. Virus-specific RNA in cells productively infected or transformed by polyoma virus. J Mol Biol. 1966 Apr;16(2):359–373. doi: 10.1016/s0022-2836(66)80179-1. [DOI] [PubMed] [Google Scholar]
  6. Bowen J. M., Hughes R. G., Dmochowski L. Studies on the inhibition of polyoma virus replication in vitro: comparison of the effects of phenethyl alcohol, 5-iodo-2'-deoxyuridine, puromycin, and actinomycin D. Tex Rep Biol Med. 1966 Summer;24(2):143–157. [PubMed] [Google Scholar]
  7. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fried M., Pitts J. D. Replication of polyoma virus DNA. I. A resting cell system for biochemical studies on polyoma virus. Virology. 1968 Apr;34(4):761–770. doi: 10.1016/0042-6822(68)90097-4. [DOI] [PubMed] [Google Scholar]
  9. Gershon D., Hausen P., Sachs L., Winocour E. On the mechanism of polyoma virus-induced synthesis of cellular DNA. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1584–1592. doi: 10.1073/pnas.54.6.1584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Habel K. Virus tumor antigens: specific fingerprints? Cancer Res. 1966 Sep;26(9):2018–2024. [PubMed] [Google Scholar]
  11. MINOWADA J. DEOXYRIBONUCLEIC ACID AND PROTEIN SYNTHESES IN POLYOMA VIRUS-INFECTED MOUSE KIDNEY CELLS IN CULTURE AS STUDIED BY AUTORADIOGRAPHY AND IMMUNOFLUORESCENCE. Gan. 1964 Aug;55:267–276. [PubMed] [Google Scholar]
  12. MINOWADA J. EFFECT OF X-IRRADIATION ON DNA SYNTHESIS IN POLYOMA VIRUS-INFECTED CULTURES. Exp Cell Res. 1964 Jan;33:161–175. doi: 10.1016/s0014-4827(64)81024-7. [DOI] [PubMed] [Google Scholar]
  13. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUMEL Action of actinomycin D on animal cells and viruses. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1238–1245. doi: 10.1073/pnas.48.7.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  15. Vogt M., Dulbecco R., Smith B. Induction of cellular DNA synthesis by polyoma virus. 3. Induction in productively infected cells. Proc Natl Acad Sci U S A. 1966 Apr;55(4):956–960. doi: 10.1073/pnas.55.4.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  17. Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winocour E., Kaye A. M., Stollar V. Synthesis and transmethylation of DNA in polyoma-infected cultures. Virology. 1965 Oct;27(2):156–169. doi: 10.1016/0042-6822(65)90155-8. [DOI] [PubMed] [Google Scholar]

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