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. 1969 Jul;4(1):15–23. doi: 10.1128/jvi.4.1.15-23.1969

Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells

K I Berns 1, C Silverman 1, A Weissbach 1,1
PMCID: PMC375832  PMID: 5804948

Abstract

HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner as the HeLa DNA polymerase. In addition to chromatographic differences, the two enzymes differed with regard to primer response, relative activity at high pH, inactivation by heat and p-chloromercuribenzoate, and inhibition by vaccinia antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  2. Geiduschek E. P., Sklar J. Continual requirement for a host RNA polymerase component in a bacteriophage development. Nature. 1969 Mar 1;221(5183):833–836. doi: 10.1038/221833a0. [DOI] [PubMed] [Google Scholar]
  3. Joklik W. K. The poxviruses. Bacteriol Rev. 1966 Mar;30(1):33–66. doi: 10.1128/br.30.1.33-66.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jungwirth C., Joklik W. K. Studies on "early" enzymes in HeLa cells infected with vaccinia virus. Virology. 1965 Sep;27(1):80–93. doi: 10.1016/0042-6822(65)90145-5. [DOI] [PubMed] [Google Scholar]
  5. Magee W. E., Miller O. V. Immunological evidence for the appearance of a new DNA-polymerase in cells infected with vaccinia virus. Virology. 1967 Jan;31(1):64–69. doi: 10.1016/0042-6822(67)90008-6. [DOI] [PubMed] [Google Scholar]
  6. Magee W. E., Miller O. V. Initiation of vaccinia virus infection in actinomycin D-pretreated cells. J Virol. 1968 Jul;2(7):678–685. doi: 10.1128/jvi.2.7.678-685.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moss B., Salzman N. P. Sequential protein synthesis following vaccinia virus infection. J Virol. 1968 Oct;2(10):1016–1027. doi: 10.1128/jvi.2.10.1016-1027.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PRICER W. E., Jr, WEISSBACH A. THE EFFECT OF LYSOGENIC INDUCTION WITH MITOMYCIN C ON THE DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI K12-LAMBDA. J Biol Chem. 1964 Aug;239:2607–2612. [PubMed] [Google Scholar]
  9. SALZMAN N. P., SHATKIN A. J., SEBRING E. D. Viral protein and DNA synthesis in vaccinia virus-infected HeLacell cultures. Virology. 1963 Apr;19:542–550. doi: 10.1016/0042-6822(63)90049-7. [DOI] [PubMed] [Google Scholar]
  10. Salzman N. P., Sebring E. D. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J Virol. 1967 Feb;1(1):16–23. doi: 10.1128/jvi.1.1.16-23.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. YONEDA M., BOLLUM F. J. DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES. J Biol Chem. 1965 Aug;240:3385–3391. [PubMed] [Google Scholar]

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