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. 1969 Aug;4(2):154–161. doi: 10.1128/jvi.4.2.154-161.1969

Ribonucleic Acid Synthesis of Vesicular Stomatitis Virus

I. Species of Ribonucleic Acid Found in Chinese Hamster Ovary Cells Infected with Plaque-forming and Defective Particles

Martha Stampfer 1, David Baltimore 1, Alice S Huang 1
PMCID: PMC375850  PMID: 4308915

Abstract

Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Girard M. An intermediate in the synthesis of poliovirus RNA. Proc Natl Acad Sci U S A. 1966 Aug;56(2):741–748. doi: 10.1073/pnas.56.2.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D. Purification and properties of poliovirus double-stranded ribonucleic acid. J Mol Biol. 1966 Jul;18(3):421–428. doi: 10.1016/s0022-2836(66)80034-7. [DOI] [PubMed] [Google Scholar]
  3. Brown F., Martin S. J., Cartwright B., Crick J. The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus. J Gen Virol. 1967 Oct;1(4):479–486. doi: 10.1099/0022-1317-1-4-479. [DOI] [PubMed] [Google Scholar]
  4. HACKETT A. J. A POSSIBLE MORPHOLOGIC BASIS FOR THE AUTOINTERFERENCE PHENOMENON IN VESICULAR STOMATITIS VIRUS. Virology. 1964 Sep;24:51–59. doi: 10.1016/0042-6822(64)90147-3. [DOI] [PubMed] [Google Scholar]
  5. Hackett A. J., Schaffer F. L., Madin S. H. The separation of infectious and autointerfering particles in vesicular stomatitis virus preparations. Virology. 1967 Jan;31(1):114–119. doi: 10.1016/0042-6822(67)90014-1. [DOI] [PubMed] [Google Scholar]
  6. Huang A. S., Greenawalt J. W., Wagner R. R. Defective T particles of vesicular stomatitis virus. I. Preparation, morphology, and some biologic properties. Virology. 1966 Oct;30(2):161–172. doi: 10.1016/0042-6822(66)90092-4. [DOI] [PubMed] [Google Scholar]
  7. Huang A. S., Wagner R. R. Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. J Mol Biol. 1966 Dec 28;22(2):381–384. doi: 10.1016/0022-2836(66)90143-4. [DOI] [PubMed] [Google Scholar]
  8. Huang A. S., Wagner R. R. Defective T particles of vesicular stomatitis virus. II. Biologic role in homologous interference. Virology. 1966 Oct;30(2):173–181. doi: 10.1016/0042-6822(66)90093-6. [DOI] [PubMed] [Google Scholar]
  9. Huang A. S., Wagner R. R. Inhibition of cellular RNA synthesis by nonreplicating vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1579–1584. doi: 10.1073/pnas.54.6.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. J Virol. 1969 Apr;3(4):404–413. doi: 10.1128/jvi.3.4.404-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
  12. Mills D. R., Peterson R. L., Spiegelman S. An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule. Proc Natl Acad Sci U S A. 1967 Jul;58(1):217–224. doi: 10.1073/pnas.58.1.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PUCK T. T., SANDERS P., PETERSEN D. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. II. CELLS FROM THE CHINESE HAMSTER OVARY GROWN IN SUSPENSION CULTURE. Biophys J. 1964 Nov;4:441–450. doi: 10.1016/s0006-3495(64)86794-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Penman S., Scherrer K., Becker Y., Darnell J. E. POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):654–662. doi: 10.1073/pnas.49.5.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schaffer F. L., Hackett A. J., Soergel M. E. Vesicular stomatitis virus RNA: complementarity between infected cell RNA and RNA's from infectious and autointerfering viral fractions. Biochem Biophys Res Commun. 1968 Jun 10;31(5):685–692. doi: 10.1016/0006-291x(68)90616-5. [DOI] [PubMed] [Google Scholar]
  16. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  17. Wagner R. R., Schnaitman T. A., Snyder R. M. Structural proteins of vesicular stomatitis viruses. J Virol. 1969 Apr;3(4):395–403. doi: 10.1128/jvi.3.4.395-403.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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