Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1969 Sep;4(3):244–251. doi: 10.1128/jvi.4.3.244-251.1969

Cells Persistently Infected with Newcastle Disease Virus

I. Properties of Mutants Isolated from Persistently Infected L Cells

Harshad Thacore 1, Julius S Youngner 1
PMCID: PMC375865  PMID: 16789100

Abstract

The strain of Newcastle disease virus (NDVpi) present in persistently infected L cells differed markedly from the Herts strain (NDV0) used to initiate the infection. NDVpi produced small plaques (less than 1 mm) in chick embryo cell cultures, whereas the wild type (NDV0) produced large plaques (2 to 3 mm). The two viruses differed in a number of additional properties. Whereas 80% of adsorbed NDV0 eluted from chicken red blood cells at 37 C, only about 20% of NDVpi was recovered under similar conditions. There was no significant difference in the neuraminidase content of the two viruses. The infectivity of NDV0 was stable for 1 hr at 48 C, whereas 99.9% of the infectivity of NDVpi was destroyed. The two viruses also differed in lethality for chick embryos; NDVpi had significantly reduced lethality for 9-day-old chick embryos when compared to NDV0. In contrast to NDV0, which produced an abortive infection in L cells, NDVpi not only replicated effectively and destroyed these cells, but also induced significantly higher quantities of interferon than did NDV0. These data furnished additional evidence for the lack of relationship of interferon production to abortive infection of L cells with NDV0. In contrast, interferon was found to play a significant role in the maintenance of persistent infection.

Full text

PDF
244

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DEINHARDT F., BERGS V. V., HENLE G., HENLE W. Studies on persistent infections of tissue cultures. III. Some quantitative aspects of host cell-virus interactions. J Exp Med. 1958 Oct 1;108(4):573–589. doi: 10.1084/jem.108.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HENLE G., DEINHARDT F., BERGS V. V., HENLE W. Studies on persistent infections of tissue cultures. I. General aspects of the system. J Exp Med. 1958 Oct 1;108(4):537–560. doi: 10.1084/jem.108.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOWE C., ROSE H. M., LEE L. T. Observations on the relationship between hemagglutinin and neuraminidase of influenza viruses. Proc Soc Exp Biol Med. 1961 Nov;108:420–425. doi: 10.3181/00379727-108-26956. [DOI] [PubMed] [Google Scholar]
  4. Laver W. G., Kilbourne E. D. Identification in a recombinant influenza virus of structural proteins derived from both parents. Virology. 1966 Nov;30(3):493–501. doi: 10.1016/0042-6822(66)90125-5. [DOI] [PubMed] [Google Scholar]
  5. Paucker K., Boxaca M. Cellular resistance to induction of interferon. Bacteriol Rev. 1967 Jun;31(2):145–156. doi: 10.1128/br.31.2.145-156.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SETO J. T., HICKEY B. J., RASMUSSEN A. F., Jr Sialidase activity and related properties of influenza A2 viruses. Virology. 1959 Dec;9:598–611. doi: 10.1016/0042-6822(59)90151-5. [DOI] [PubMed] [Google Scholar]
  7. Schloer G. M., Hanson R. P. Relationship of plaque size and virulence for chickens of 14 representative Newcastle disease virus strains. J Virol. 1968 Jan;2(1):40–47. doi: 10.1128/jvi.2.1.40-47.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tsvetkova I. V., Lipkind M. A. Studies on the role of myxovirus neuraminidase in virus-cell receptors interaction by means of direct determination of sialic acid split from cells. I. Experiments on influenza virus-RBC cell system. Arch Gesamte Virusforsch. 1968;23(4):299–312. doi: 10.1007/BF01242126. [DOI] [PubMed] [Google Scholar]
  9. Youngner J. S., Hallum J. V. Inhibition of induction of interferon synthesis in L-cells pretreated with interferon. Virology. 1969 Mar;37(3):473–475. doi: 10.1016/0042-6822(69)90231-1. [DOI] [PubMed] [Google Scholar]
  10. Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J Bacteriol. 1966 Oct;92(4):862–868. doi: 10.1128/jb.92.4.862-868.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Youngner J. S., Scott A. W. Relation of interferon synthesis to abortive replication of Newcastle disease virus in L cells. J Virol. 1968 Jan;2(1):81–82. doi: 10.1128/jvi.2.1.81-82.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES