Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1969 Sep;4(3):283–291. doi: 10.1128/jvi.4.3.283-291.1969

Precursor Products Found in Formaldehyde-fixed Lysates of BHK-21 Cells Infected with Pseudorabies Virus

Robert J Sydiskis 1
PMCID: PMC375870  PMID: 16789105

Abstract

Lysates of BHK-21 cells, infected with pseudorabies and labeled with 3H-thymidine, were treated with formaldehyde and centrifuged in preformed CsCl gradients. Five peaks, designated bands A to E, were detected and shown to contain the following virus-specific products: band A, aggregates of virions; band B, virions; band C, empty capsids and nucleocapsids; bands D and E, small nucleoid-like particles. All five bands contained viral deoxyribonucleic acid and virous-specific antigens. Deoxyribonuclease treatment of the lysates before centrifugation resulted in the complete loss of 3H-thymidine-labeled material from bands D and E; bands B and C were unaffected. Pulse-chase studies showed that 3H-thymidine can be rapidly chased into the virus-specific products sedimenting in bands D and E. Further chase resulted in the gradual loss of label from bands D and E with a concomitant increase in the amount of label in bands A, B, and C. These results indicate that the virus-specific products in bands D and E are precursors of pseudorabies virions and may represent the nucleoid of the virion.

Full text

PDF
283

Images in this article

287Image
on p.287

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKER P., MELNICK J. L., MAYOR H. D. A MORPHOLOGIC COMPARISON BETWEEN THE DEVELOPMENTAL STAGES OF HERPES ZOSTER AND HUMAN CYTOMEGALOVIRUS. Exp Mol Pathol. 1965 Feb;76:11–23. doi: 10.1016/0014-4800(65)90020-1. [DOI] [PubMed] [Google Scholar]
  2. BEN-PORAT T., KAPLAN A. S. The synthesis and fate of pseudorabies virus DNA in infected mammalian cells in the stationary phase of growth. Virology. 1963 Jun;20:310–317. doi: 10.1016/0042-6822(63)90120-x. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. EPSTEIN M. A. Observations on the fine structure of mature herpes simplex virus and on the composition of its nucleoid. J Exp Med. 1962 Jan 1;115:1–12. doi: 10.1084/jem.115.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EPSTEIN M. A. Observations on the mode of release of herpes virus from infected HeLa cells. J Cell Biol. 1962 Mar;12:589–597. doi: 10.1083/jcb.12.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HAMADA C., KAPLAN A. S. KINETICS OF SYNTHESIS OF VARIOUS TYPES OF ANTIGENIC PROTEINS IN CELLS INFECTED WITH PSEUDORABIES VIRUS. J Bacteriol. 1965 May;89:1328–1334. doi: 10.1128/jb.89.5.1328-1334.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KAPLAN A. S., BEN-PORAT T. The pattern of viral and cellular DNA synthesis in pseudorabies virus-infected cells in the logarithmic phase of growth. Virology. 1963 Feb;19:205–214. doi: 10.1016/0042-6822(63)90010-2. [DOI] [PubMed] [Google Scholar]
  9. Kaplan A. S., Benporat T., Kamiya T. Incorporation of 5-bromodeoxyuridine and 5-iododeoxyuridine into viral DNA and its effect on the infective process. Ann N Y Acad Sci. 1965 Jul 30;130(1):226–239. doi: 10.1111/j.1749-6632.1965.tb12556.x. [DOI] [PubMed] [Google Scholar]
  10. MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. POLLARD E. The action of ionizing radiation on viruses. Adv Virus Res. 1954;2:109–151. doi: 10.1016/s0065-3527(08)60531-x. [DOI] [PubMed] [Google Scholar]
  13. POWELL W. F. Radiosensitivity as an index of herpes simplex virus development. Virology. 1959 Sep;9:1–19. doi: 10.1016/0042-6822(59)90096-0. [DOI] [PubMed] [Google Scholar]
  14. ROIZMAN B., ROANE P. R., Jr THE MULTIPLICATION OF HERPES SIMPLEX VIRUS. II. THE RELATION BETWEEN PROTEIN SYNTHESIS AND THE DUPLICATION OF VIRAL DNA IN INFECTED HEP-2 CELLS. Virology. 1964 Feb;22:262–269. doi: 10.1016/0042-6822(64)90011-x. [DOI] [PubMed] [Google Scholar]
  15. Roizman B., Spring S. B., Roane P. R., Jr Cellular compartmentalization of herpesvirus antigens during viral replication. J Virol. 1967 Feb;1(1):181–192. doi: 10.1128/jvi.1.1.181-192.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spring S. B., Roizman B. Herpes simplex virus products in productive and abortive infection. I. Stabilization with formaldehyde and preliminary analyses by isopycnic centrifugation in CsCl. J Virol. 1967 Apr;1(2):294–301. doi: 10.1128/jvi.1.2.294-301.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spring S. B., Roizman B., Schwartz J. Herpes simplex virus products in productive and abortive infection. II. Electron microscopic and immunological evidence for failure of virus envelopment as a cause of abortive infection. J Virol. 1968 Apr;2(4):384–392. doi: 10.1128/jvi.2.4.384-392.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WATSON D. H., WILDY P., RUSSELL W. C. QUANTITATIVE ELECTRON MICROSCOPY STUDIES ON THE GROWTH OF HERPES VIRUS USING THE TECHNIQUES OF NEGATIVE STAINING AND ULTRAMICROTOMY. Virology. 1964 Dec;24:523–538. doi: 10.1016/0042-6822(64)90204-1. [DOI] [PubMed] [Google Scholar]
  19. WATSON D. H., WILDY P. SOME SEROLOGICAL PROPERTIES OF HERPES VIRUS PARTICLES STUDIED WITH THE ELECTRON MICROSCOPE. Virology. 1963 Sep;21:100–111. doi: 10.1016/0042-6822(63)90308-8. [DOI] [PubMed] [Google Scholar]
  20. WILDY P., RUSSELL W. C., HORNE R. W. The morphology of herpes virus. Virology. 1960 Oct;12:204–222. doi: 10.1016/0042-6822(60)90195-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES