Abstract
Lysates of BHK-21 cells, infected with pseudorabies and labeled with 3H-thymidine, were treated with formaldehyde and centrifuged in preformed CsCl gradients. Five peaks, designated bands A to E, were detected and shown to contain the following virus-specific products: band A, aggregates of virions; band B, virions; band C, empty capsids and nucleocapsids; bands D and E, small nucleoid-like particles. All five bands contained viral deoxyribonucleic acid and virous-specific antigens. Deoxyribonuclease treatment of the lysates before centrifugation resulted in the complete loss of 3H-thymidine-labeled material from bands D and E; bands B and C were unaffected. Pulse-chase studies showed that 3H-thymidine can be rapidly chased into the virus-specific products sedimenting in bands D and E. Further chase resulted in the gradual loss of label from bands D and E with a concomitant increase in the amount of label in bands A, B, and C. These results indicate that the virus-specific products in bands D and E are precursors of pseudorabies virions and may represent the nucleoid of the virion.
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Selected References
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- BECKER P., MELNICK J. L., MAYOR H. D. A MORPHOLOGIC COMPARISON BETWEEN THE DEVELOPMENTAL STAGES OF HERPES ZOSTER AND HUMAN CYTOMEGALOVIRUS. Exp Mol Pathol. 1965 Feb;76:11–23. doi: 10.1016/0014-4800(65)90020-1. [DOI] [PubMed] [Google Scholar]
- BEN-PORAT T., KAPLAN A. S. The synthesis and fate of pseudorabies virus DNA in infected mammalian cells in the stationary phase of growth. Virology. 1963 Jun;20:310–317. doi: 10.1016/0042-6822(63)90120-x. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- EPSTEIN M. A. Observations on the fine structure of mature herpes simplex virus and on the composition of its nucleoid. J Exp Med. 1962 Jan 1;115:1–12. doi: 10.1084/jem.115.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EPSTEIN M. A. Observations on the mode of release of herpes virus from infected HeLa cells. J Cell Biol. 1962 Mar;12:589–597. doi: 10.1083/jcb.12.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAMADA C., KAPLAN A. S. KINETICS OF SYNTHESIS OF VARIOUS TYPES OF ANTIGENIC PROTEINS IN CELLS INFECTED WITH PSEUDORABIES VIRUS. J Bacteriol. 1965 May;89:1328–1334. doi: 10.1128/jb.89.5.1328-1334.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN A. S., BEN-PORAT T. The pattern of viral and cellular DNA synthesis in pseudorabies virus-infected cells in the logarithmic phase of growth. Virology. 1963 Feb;19:205–214. doi: 10.1016/0042-6822(63)90010-2. [DOI] [PubMed] [Google Scholar]
- Kaplan A. S., Benporat T., Kamiya T. Incorporation of 5-bromodeoxyuridine and 5-iododeoxyuridine into viral DNA and its effect on the infective process. Ann N Y Acad Sci. 1965 Jul 30;130(1):226–239. doi: 10.1111/j.1749-6632.1965.tb12556.x. [DOI] [PubMed] [Google Scholar]
- MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POLLARD E. The action of ionizing radiation on viruses. Adv Virus Res. 1954;2:109–151. doi: 10.1016/s0065-3527(08)60531-x. [DOI] [PubMed] [Google Scholar]
- POWELL W. F. Radiosensitivity as an index of herpes simplex virus development. Virology. 1959 Sep;9:1–19. doi: 10.1016/0042-6822(59)90096-0. [DOI] [PubMed] [Google Scholar]
- ROIZMAN B., ROANE P. R., Jr THE MULTIPLICATION OF HERPES SIMPLEX VIRUS. II. THE RELATION BETWEEN PROTEIN SYNTHESIS AND THE DUPLICATION OF VIRAL DNA IN INFECTED HEP-2 CELLS. Virology. 1964 Feb;22:262–269. doi: 10.1016/0042-6822(64)90011-x. [DOI] [PubMed] [Google Scholar]
- Roizman B., Spring S. B., Roane P. R., Jr Cellular compartmentalization of herpesvirus antigens during viral replication. J Virol. 1967 Feb;1(1):181–192. doi: 10.1128/jvi.1.1.181-192.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spring S. B., Roizman B. Herpes simplex virus products in productive and abortive infection. I. Stabilization with formaldehyde and preliminary analyses by isopycnic centrifugation in CsCl. J Virol. 1967 Apr;1(2):294–301. doi: 10.1128/jvi.1.2.294-301.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spring S. B., Roizman B., Schwartz J. Herpes simplex virus products in productive and abortive infection. II. Electron microscopic and immunological evidence for failure of virus envelopment as a cause of abortive infection. J Virol. 1968 Apr;2(4):384–392. doi: 10.1128/jvi.2.4.384-392.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON D. H., WILDY P., RUSSELL W. C. QUANTITATIVE ELECTRON MICROSCOPY STUDIES ON THE GROWTH OF HERPES VIRUS USING THE TECHNIQUES OF NEGATIVE STAINING AND ULTRAMICROTOMY. Virology. 1964 Dec;24:523–538. doi: 10.1016/0042-6822(64)90204-1. [DOI] [PubMed] [Google Scholar]
- WATSON D. H., WILDY P. SOME SEROLOGICAL PROPERTIES OF HERPES VIRUS PARTICLES STUDIED WITH THE ELECTRON MICROSCOPE. Virology. 1963 Sep;21:100–111. doi: 10.1016/0042-6822(63)90308-8. [DOI] [PubMed] [Google Scholar]
- WILDY P., RUSSELL W. C., HORNE R. W. The morphology of herpes virus. Virology. 1960 Oct;12:204–222. doi: 10.1016/0042-6822(60)90195-1. [DOI] [PubMed] [Google Scholar]