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. 1969 Oct;4(4):364–371. doi: 10.1128/jvi.4.4.364-371.1969

Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli

I. Location of “Late” MS2-Specific Ribonucleic Acid Synthesis

Anne M Haywood 1,2, Jane Harris Cramer 1,2,1, Nadja Lancaster Shoemaker 1,2,2
PMCID: PMC375883  PMID: 4980849

Abstract

When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 × g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 × g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg2+. In the presence of high Mg+, MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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