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. 1969 Oct;4(4):400–407. doi: 10.1128/jvi.4.4.400-407.1969

φX-174 Bacteriophage Structural Mutants Which Affect Deoxyribonucleic Acid Synthesis

Jeff E D Siegel 1,1, Masaki Hayashi 1
PMCID: PMC375888  PMID: 5823229

Abstract

Seven cistrons in φX-174 were identified and one in particular was studied intensively: cistron A, which is assigned a protein in the mature phage. Amber mutants in this cistron synthesize a new deoxyribonucleic acid (DNA) form in addition to circular phage DNA upon infection of the restrictive host. This DNA is linear, non-infectious, and single-stranded; it is formed from the phage strand of replicative form φX-174 DNA. These mutants produce two different defective particles in the restrictive host. One particle contains circular phage DNA but is not infectious; the other contains the new DNA form and is similar to the 70S particles found in wild-type phage lysates. The mutant A gene product acts independently of normal A protein upon mixed infection of the restrictive host with an A mutant and a mutant from any other cistron or wild type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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