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Properties of secondary fibroblast (2°F) reprogramming. FACS analysis establishing the dynamics of SSEA1 activation in 2°F/1B and 2°F/6C cell lines. The inset shows colony formation as early as d5. On d6 of reprogramming gene induction, the cultures were also passed by standard trypsinization and analyzed in parallel to eliminate the negative effect of cell overgrowth.
