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. 1969 Nov;4(5):567–573. doi: 10.1128/jvi.4.5.567-573.1969

Serological Relatedness of the Ribonucleic Acid-Containing Coliphages

Robert G Krueger 1,2
PMCID: PMC375910  PMID: 5360520

Abstract

The serological relationship of the ribonucleic acid (RNA)-containing coliphages MS-2, M-12, R-17, f2, β, fr, f4, and Qβ was determined. Antisera against MS-2, R-17, f2, fr, and Qβ neutralized the infectivity of all of these RNA phages to varying degrees. Although each phage was serologically distinct, the antisera cross-reacted with certain phages to approximately the same degree, indicating the antigenic relationship of the coat proteins of these phages. Adsorption of anti-MS-2 sera with varying concentrations of all of the phages demonstrated that these viruses contain similar yet unique antigenic determinants. It is suggested that these RNA phages are mutants of two related phages rather than of the same phage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Enger M. D., Kaesberg P. Comparative studies of the coat proteins of R-17 and M-12 bacteriophages. J Mol Biol. 1965 Aug;13(1):260–268. doi: 10.1016/s0022-2836(65)80095-x. [DOI] [PubMed] [Google Scholar]
  2. Konigsberg W., Weber K., Notani G., Zinder N. The isolation and characterization of the tryptic peptides from the f2 bacteriophage coat protein. J Biol Chem. 1966 Jun 10;241(11):2579–2588. [PubMed] [Google Scholar]
  3. Krueger R. G. The effect of Streptomycin on antibody synthesis in vitro. Proc Natl Acad Sci U S A. 1965 Jul;54(1):144–152. doi: 10.1073/pnas.54.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Mäkelä O. Single lymph node cells producing heteroclitic bacteriophage antibody. J Immunol. 1965 Aug;95(2):378–386. [PubMed] [Google Scholar]
  6. Overby L. R., Barlow G. H., Doi R. H., Jacob M., Spiegelman S. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles. J Bacteriol. 1966 Jan;91(1):442–448. doi: 10.1128/jb.91.1.442-448.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Overby L. R., Barlow G. H., Doi R. H., Jacob M., Spiegelman S. Comparison of two serologically distinct ribonucleic acid bacteriophages. II. Properties of the nucleic acids and coat proteins. J Bacteriol. 1966 Sep;92(3):739–745. doi: 10.1128/jb.92.3.739-745.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SCOTT D. W. SEROLOGICAL CROSS REACTIONS AMONG THE RNA-CONTAINING COLIPHAGES. Virology. 1965 May;26:85–88. doi: 10.1016/0042-6822(65)90028-0. [DOI] [PubMed] [Google Scholar]
  9. STRAUSS J. H., Jr, SINSHEIMER R. L. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J Mol Biol. 1963 Jul;7:43–54. doi: 10.1016/s0022-2836(63)80017-0. [DOI] [PubMed] [Google Scholar]
  10. Vasquez C., Granboulan N., Franklin R. M. Structure of the ribonucleic acid bacteriophage R17. J Bacteriol. 1966 Dec;92(6):1779–1786. doi: 10.1128/jb.92.6.1779-1786.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weber K., Notani G., Wikler M., Konigsberg W. Amino acid sequence of the f2 coat protein. J Mol Biol. 1966 Sep;20(2):423–425. doi: 10.1016/0022-2836(66)90073-8. [DOI] [PubMed] [Google Scholar]
  12. Wittmann-Liebold B. Die Proteinstruktur des RNA-Bakteriophagen f-r. I. Aminosäurezusammensetzung, tryptische Peptide und Partialsequenzen. Z Vererbungsl. 1965;97(3):272–280. [PubMed] [Google Scholar]

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