Figure 1.

NeuroD1 is expressed in aggressive neuroendocrine lung cancers. (a) Expression of NeuroD1 mRNA compared across diverse normal (N=3879; black dots) and malignant tissues (N=1605; red dots) using the Affymetrix HGU133 Plus v2 GeneChip. (b, d) Melanoma and prostate cancer cell lines were lysed, and 25 μg total protein was loaded and then immunoblotted for NeuroD1, TrkB, NCAM, synaptophysin and tubulin or GAPDH (as loading controls). Melanoma cell lines were loaded by increasing pigmentation. (c) Lung cancer cell lines were lysed and 25 μg of total protein was immunoblotted for synaptophysin and tubulin (as loading controls).