Abstract
Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A “core” particle was obtained by degradation of the virion with deoxycholate at 0 C; it contained the viral ribonucleic acid and the two small structural proteins. The “envelope” material released from around the core was identified with the most prominent (intermediate) protein seen in electropherograms of virion proteins. In addition to the structural proteins, at least three additional proteins (specified by virus infection) were found in the cytoplasm. The cytoplasmic proteins associated with Kunjin virus differed in their electrophoretic profile from those associated with infection by the related Murray Valley encephalitis virus.
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Selected References
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- Burge B. W., Pfefferkorn E. R. Functional defects of temperature-sensitive mutants of Sindbis virus. J Mol Biol. 1968 Jul 14;35(1):193–205. doi: 10.1016/s0022-2836(68)80047-6. [DOI] [PubMed] [Google Scholar]
- DOHERTY R. L., CARLEY J. G., MACKERRAS M. J., MARKS E. N. Studies of arthropod-borne virus infections in Queensland. III. Isolation and characterization of virus strains from wild-caught mosquitoes in North Queensland. Aust J Exp Biol Med Sci. 1963 Feb;41:17–39. doi: 10.1038/icb.1963.2. [DOI] [PubMed] [Google Scholar]
- Friedman R. M. Structural and nonstructural proteins of an arbovirus. J Virol. 1968 Oct;2(10):1076–1080. doi: 10.1128/jvi.2.10.1076-1080.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hay A. J., Skehel J. J., Burke D. C. Proteins synthesized in chick cells following infection with Semliki Forest virus. J Gen Virol. 1968 Sep;3(2):175–184. doi: 10.1099/0022-1317-3-2-175. [DOI] [PubMed] [Google Scholar]
- Holland J. J., Kiehn E. D. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015–1022. doi: 10.1073/pnas.60.3.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
- Maizel J. V., Jr, Summers D. F. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells. Virology. 1968 Sep;36(1):48–54. doi: 10.1016/0042-6822(68)90115-3. [DOI] [PubMed] [Google Scholar]
- Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
- Mass M., Fine R., Murakami W. T. Protein composition of polyoma virus. J Mol Biol. 1968 Aug 28;36(1):167–177. doi: 10.1016/0022-2836(68)90227-1. [DOI] [PubMed] [Google Scholar]
- Sreevalsan T., Allen P. T. Replication of Western equine encephalomyelitis virus. II. Cytoplasmic structure involved in the synthesis and development of the virions. J Virol. 1968 Oct;2(10):1038–1046. doi: 10.1128/jvi.2.10.1038-1046.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stollar V., Stevens T. M., Schlesinger R. W. Studies on the nature of dengue viruses. II. Characterization of viral RNA and effects of inhibitors of RNA synthesis. Virology. 1966 Oct;30(2):303–312. doi: 10.1016/0042-6822(66)90105-x. [DOI] [PubMed] [Google Scholar]
- Strauss J. H., Jr, Burge B. W., Darnell J. E. Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology. 1969 Mar;37(3):367–376. doi: 10.1016/0042-6822(69)90220-7. [DOI] [PubMed] [Google Scholar]
- Strauss J. H., Jr, Burge B. W., Pfefferkorn E. R., Darnell J. E., Jr Identification of the membrane protein and "core" protein of Sindbis virus. Proc Natl Acad Sci U S A. 1968 Feb;59(2):533–537. doi: 10.1073/pnas.59.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr Evidence for large precursor proteins in poliovirus synthesis. Proc Natl Acad Sci U S A. 1968 Mar;59(3):966–971. doi: 10.1073/pnas.59.3.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Westaway E. G. Antibody responses in rabbits to the group B arbovirus Kumjin: serologic activity of the fractionated immunoglobulins in homologous and heterologous reactions. J Immunol. 1968 Mar;100(3):569–580. [PubMed] [Google Scholar]
- Westaway E. G. Assessment and application of a cell line from pig kidney for plaque assay and neutralization tests with twelve group B arboviruses. Am J Epidemiol. 1966 Nov;84(3):439–456. doi: 10.1093/oxfordjournals.aje.a120657. [DOI] [PubMed] [Google Scholar]
- Yin F. H., Lockart R. Z., Jr Maturation defects in temperature-sensitive mutants of Sindbis virus. J Virol. 1968 Jul;2(7):728–737. doi: 10.1128/jvi.2.7.728-737.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]