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. 1969 Nov;4(5):694–703. doi: 10.1128/jvi.4.5.694-703.1969

In Vitro Transformation by Adenovirus-Simian Virus 40 Hybrid Viruses

IV. Properties of Clones Isolated from Cell Lines Transformed by Adenovirus 2-Simian Virus 40 and Adenovirus 12-Simian Virus 40 Transcapsidant Hybrid Viruses

Paul H Black 1,2, Leonard D Berman 1,2, Cynthia B Dixon 1,2
PMCID: PMC375927  PMID: 4311797

Abstract

Clones were isolated from hamster cells transformed by the adenovirus 2-SV40 and adenovirus 12-SV40 transcapsidant hybrid viruses. The clones were characterized with respect to their cytomorphology, virus and antigen content, and the histomorphology of tumors induced by transplantation of the clonal sublines to hamsters. Three different cellular and colonial morphologies were observed. Clones with an SV40 morphology gave rise to tumors predominantly with an SV40 histology, whereas clones with an adenovirus morphology produced typical adenovirus tumors upon transplantation of the transformed cells. Clones which had features of both SV40 and adenovirus transformed cells gave rise to “intermediate” and adenovirus tumors. The results indicate that multiple events occur during transformation and tumorigenesis by the transcapsidant virus populations and provide an explanation for the multiplicity of findings which have been reported with these virus populations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK P. H., ROWE W. P., COOPER H. L. AN ANALYSIS OF SV 40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO. II. STUDIES OF THREE CLONES DERIVED FROM A CONTINUOUS LINE OF TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Nov;50:847–854. doi: 10.1073/pnas.50.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYER G. S., DENNY F. W., Jr, GINSBERG H. S. Sequential cellular changes produced by types 5 and 7 adenoviruses in HeLa cells and in human amniotic cells; cytological studies aided by fluorescein-labelled antibody. J Exp Med. 1959 Nov 1;110:827–844. doi: 10.1084/jem.110.5.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berman L. D. Comparative morphologic study of the virus-induced solid tumors of Syrian hamsters. J Natl Cancer Inst. 1967 Nov;39(5):847–901. [PubMed] [Google Scholar]
  4. Black P. H., Lewis A. M., Jr, Blacklow N. R., Austin J. B., Rowe W. P. The presence of adenovirus-specific antigens in hamster cells rendered neoplastic by adenovirus 1-SV40 and adenovirus 2-SV40 hybrid viruses. Proc Natl Acad Sci U S A. 1967 May;57(5):1324–1330. doi: 10.1073/pnas.57.5.1324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Black P. H., Todaro G. J. In vitro transformation of hamster and human cells with the adeno 7-SV 40 hybrid virus. Proc Natl Acad Sci U S A. 1965 Aug;54(2):374–381. doi: 10.1073/pnas.54.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Black P. H., White B. J. In vitro transformation by the adenovirus-SV40 hybrid viruses. II. Characteristics of the transformation of hamster cells by the adeno 2-, adeno 3-, and adeno 12-SV40 viruses. J Exp Med. 1967 Apr 1;125(4):629–646. doi: 10.1084/jem.125.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Butel J. S., Rapp F. Inactivation and density studies with PARA-adenovirus 7 (SV40-adenovirus "hybrid" population). J Immunol. 1966 Oct;97(4):546–553. [PubMed] [Google Scholar]
  8. Freeman A. E., Hollinger S., Price P. J., Calisher C. The effect of calcium on cell lines derived from adenovirus type 12-induced hamster tumors. Exp Cell Res. 1965 Aug;39(1):259–264. doi: 10.1016/0014-4827(65)90028-5. [DOI] [PubMed] [Google Scholar]
  9. HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Igel H. J., Black P. H. In vitro transformation by the adenovirus-SV40 hybrid viruses. 3. Morphology of tumors induced with transformed cells. J Exp Med. 1967 Apr 1;125(4):647–656. doi: 10.1084/jem.125.4.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kirchstein R. L., Rabson A. S., O'Conor G. T. Ependymomas produced in Syrian hamsters by adenovirus 7, strain E46 ("hybrid" of adenovirus 7 and SV40). Proc Soc Exp Biol Med. 1965 Nov;120(2):484–487. doi: 10.3181/00379727-120-30568. [DOI] [PubMed] [Google Scholar]
  12. Lausch R. N., Tevethia S. S., Rapp F. Evidence of SV40-specific transplantation and surface antigens in cells transformed by PARA-adenovirus 12. J Immunol. 1968 Oct;101(4):645–649. [PubMed] [Google Scholar]
  13. Levin M. J., Black P. H., Coghill S. L., Dixon C. B., Henry P. H. In vitro transformation by the adenovirus-simian virus 40 hybrid viruses. V. Virus-specific ribonucleic acid in cell lines transformed by the adenovirus 2-simian virus 40 and adenovirus 12-simian virus 40 transcapsidant hybrid viruses. J Virol. 1969 Nov;4(5):704–711. doi: 10.1128/jvi.4.5.704-711.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lewis A. M., Jr, Baum S. G., Prigge K. O., Rowe W. P. Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc Soc Exp Biol Med. 1966 May;122(1):214–218. doi: 10.3181/00379727-122-31093. [DOI] [PubMed] [Google Scholar]
  15. Lewis A. M., Wiese W. H., Rowe W. P. The presence of antibodies in human serum to early (T) adenovirus antigens. Proc Natl Acad Sci U S A. 1967 Mar;57(3):622–629. doi: 10.1073/pnas.57.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  17. McAllister R. M., Reed G., Huebner R. J. Colonial growth in agar of cells derived from adenovirus-induced hamster tumors. J Natl Cancer Inst. 1967 Jul;39(1):43–53. [PubMed] [Google Scholar]
  18. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. RAPP F., MELNICK J. L., BUTEL J. S., KITAHARA T. THE INCORPORATION OF SV40 MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGEN. Proc Natl Acad Sci U S A. 1964 Dec;52:1348–1352. doi: 10.1073/pnas.52.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rabson A. S., Malmgren R. A., Kirschstein R. L. Induction of neoplasia in vitro in hamster kidney tissue by adenovirus 7-SV40 "hybrid" strain (LLE46). Proc Soc Exp Biol Med. 1966 Feb;121(2):486–489. doi: 10.3181/00379727-121-30811. [DOI] [PubMed] [Google Scholar]
  24. Rapp F., Butel J. S., Melnick J. L. SV40-adenovirus "hybrid" populations: transfer of SV40 determinants from one type of adenovirus to another. Proc Natl Acad Sci U S A. 1965 Sep;54(3):717–724. doi: 10.1073/pnas.54.3.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rapp F., Butel J. S., Tevethia S. S., Katz M., Melnick J. L. Antigenic analysis of tumors and sera from aimals inoculated with para-adenovirus populations. J Immunol. 1966 Dec;97(6):833–839. [PubMed] [Google Scholar]
  26. Rapp F., Melnick J. L., Levy B. Correlation of immunology and histopathology of tumors induced by defective SV40-adenovirus hybrids. Am J Pathol. 1967 May;50(5):849–859. [PMC free article] [PubMed] [Google Scholar]
  27. Robertson H. T., Black P. H. Changes in surface antigens of SV40-virus transformed cells. Proc Soc Exp Biol Med. 1969 Feb;130(2):363–370. doi: 10.3181/00379727-130-33556. [DOI] [PubMed] [Google Scholar]
  28. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rowe W. P., Pugh W. E. Studies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials. Proc Natl Acad Sci U S A. 1966 May;55(5):1126–1132. doi: 10.1073/pnas.55.5.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rowe W. P. Studies of adenovirus-SV40 hybrid viruses. 3. Transfer of SV40 gene between adenovirus types. Proc Natl Acad Sci U S A. 1965 Sep;54(3):711–717. doi: 10.1073/pnas.54.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Strohl W. A., Rabson A. S., Rouse H. Adenovirus tumorigenesis: role of the viral genome in determining tumor morphology. Science. 1967 Jun 23;156(3782):1631–1633. doi: 10.1126/science.156.3782.1631. [DOI] [PubMed] [Google Scholar]
  33. TEMIN H. M. The control of cellular morphology in embryonic cells infected with rous sarcoma virus in vitro. Virology. 1960 Feb;10:182–197. doi: 10.1016/0042-6822(60)90038-6. [DOI] [PubMed] [Google Scholar]
  34. Wells S. A., Jr, Orme S. K., Rabson A. S. Induction of neoplasia in vitro in hamster thyroid tissue by SV40 and adenovirus 7-SV40 "hybrid" (STRAIN LLE46). Proc Soc Exp Biol Med. 1966 Nov;123(2):507–510. doi: 10.3181/00379727-123-31528. [DOI] [PubMed] [Google Scholar]
  35. Wells S. A., Jr, Rabson A. S., Malmgren R. A., Ketchmam A. S. In vitro neoplastic transformation of newborn hamster salivary-gland tissue by oncogenic DNA viruses. Cancer. 1966 Oct;19(10):1411–1415. doi: 10.1002/1097-0142(196610)19:10<1411::aid-cncr2820191012>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]

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