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. 1969 Nov;4(5):719–726. doi: 10.1128/jvi.4.5.719-726.1969

Polynucleotide Ligase Activity in Cells Infected with Simian Virus 40, Polyoma Virus, or Vaccinia Virus

J Sambrook 1,1, A J Shatkin 1,2
PMCID: PMC375930  PMID: 4311800

Abstract

The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infection of mouse embryo, monkey kidney, and HeLa cells with polyoma virus, SV40, and vaccinia virus, respectively, the enzyme activity increased, but its cofactor requirement was unchanged. In vaccinia virus-infected cells, the increased activity was localized in the cytoplasm. Ligase induction occurred in the presence of cytosine arabinoside but was prevented by puromycin. Rifampicin blocked the production of infectious vaccinia particles but had little effect on the induction of ligase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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