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. 1969 Nov;4(5):763–768. doi: 10.1128/jvi.4.5.763-768.1969

Biophysical Properties of Australia Antigen

J L Gerin 1,2, R H Purcell 1,2, MD Hoggan 1,2, P V Holland 1,2, R M Chanock 1,2
PMCID: PMC375935  PMID: 5360526

Abstract

Biophysical studies with Australia complement-fixing (CF) antigen showed it to be a particle with a buoyant density of 1.20 g/cm3 in CsCl, a sedimentation coefficient of 110, and an average diameter of 25 nm. The CF antigen was not inactivated by ether, 1% deoxycholate, 1% Tween 80 or overnight heating at 56 C. The antigen was unstable when treated with 1% sodium dodecyl sulfate. A procedure is described for the isolation and partial purification of Australia antigen from serum by using isopycnic banding and rate separation techniques. Treatment of the 1.20 g/cm3 Australia antigen with 1% Tween 80 yielded a minor peak of CF activity with a buoyant density of 1.39 g/cm3 in CsCl.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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