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. 1969 Nov;4(5):769–776. doi: 10.1128/jvi.4.5.769-776.1969

Morphogenesis of Influenza A Virus in Ehrlich Ascites Tumor Cells as Revealed by Thin-Sectioning and Freeze-Etching

T Bächi 1, W Gerhard 1, J Lindenmann 1, K Mühlethaler 1
PMCID: PMC375936  PMID: 5391162

Abstract

The budding of a tumor-adapted strain of influenza A0 virus at the surface of Ehrlich ascites tumor cells was studied by electron microscopy. Thin sections of budding sites showed the formation of a fuzzy coat on the outside of the cell membrane and simultaneously the apposition of a dark layer on the inner side. The continuity of cellular and viral membrane seemed to be preserved up to the point where the virion remained attached by only a thin stalk. Freeze-etching of virus budding sites yielded pictures in which a clear differentiation between the viral membrane and the host cell membrane was visible. The breaks across the fuzzy coat revealed striations corresponding to the “spikes” seen in negative contrast, whereas tangentially broken virus particles were best interpreted by assuming that splitting occurred midway between the two outer layers of the envelope.

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These references are in PubMed. This may not be the complete list of references from this article.

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