Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1969 Dec;4(6):866–871. doi: 10.1128/jvi.4.6.866-871.1969

Characterization of Deoxyribonucleases Induced by Poxviruses 1

C Jungwirth 1, J Launer 1, G Dombrowski 1, I Horák 1
PMCID: PMC375950  PMID: 16789119

Abstract

Increases in deoxyribonuclease activity assayed at alkaline pH can be observed in poxvirus-infected cells when native or denatured deoxyribonucleic acid (DNA) is used as substrate. The deoxyribonuclease assayable with native DNA as substrate, induced in HeLa cells by cowpoxvirus or vaccinia virus WR, can be separated from the corresponding enzyme present in normal cells by chromatography on diethylaminoethyl cellulose. In addition, the two enzymes induced in the virus-infected cells differ from each other in their chromatographic properties. The two induced enzymes have been further characterized with respect to properties of enzymatic reaction.

Full text

PDF
866

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKER Y., JOKLIK W. K. MESSENGER RNA IN CELLS INFECTED WITH VACCINIA VIRUS. Proc Natl Acad Sci U S A. 1964 Apr;51:577–585. doi: 10.1073/pnas.51.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birnboim H. The use of gel filtration to distinguish between endonucleolytic and exonucleolytic types of degradation. Biochim Biophys Acta. 1966 Apr 18;119(1):198–200. doi: 10.1016/0005-2787(66)90052-9. [DOI] [PubMed] [Google Scholar]
  4. Eron L. J., McAuslan B. R. The nature of poxvirus-induced deoxyribonucleas. Biochem Biophys Res Commun. 1966 Mar 8;22(5):518–523. doi: 10.1016/0006-291x(66)90305-6. [DOI] [PubMed] [Google Scholar]
  5. GEIDUSCHEK E. P., DANIELS A. A SIMPLE ASSAY FOR DNA ENDONUCLEASES. Anal Biochem. 1965 Apr;11:133–137. doi: 10.1016/0003-2697(65)90052-7. [DOI] [PubMed] [Google Scholar]
  6. HANAFUSA H. Factors involved in the initiation of multiplication of vaccinia virus. Cold Spring Harb Symp Quant Biol. 1962;27:209–217. doi: 10.1101/sqb.1962.027.001.021. [DOI] [PubMed] [Google Scholar]
  7. JOKLIK W. K. The purification fo four strains of poxvirus. Virology. 1962 Sep;18:9–18. doi: 10.1016/0042-6822(62)90172-1. [DOI] [PubMed] [Google Scholar]
  8. Jungwirth C., Joklik W. K. Studies on "early" enzymes in HeLa cells infected with vaccinia virus. Virology. 1965 Sep;27(1):80–93. doi: 10.1016/0042-6822(65)90145-5. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. McAuslan B. R., Herde P., Pett D., Ross J. Nucleases of virus-infected animal cells. Biochem Biophys Res Commun. 1965 Sep 8;20(5):586–591. doi: 10.1016/0006-291x(65)90439-0. [DOI] [PubMed] [Google Scholar]
  11. McAuslan B. R., Kates J. R. Regulation of virus-induced deoxyribonucleases. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1581–1587. doi: 10.1073/pnas.55.6.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morrison J. M., Keir H. M. A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme. J Gen Virol. 1968 Dec;3(3):337–347. doi: 10.1099/0022-1317-3-3-337. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES