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. 1969 Dec;4(6):890–895. doi: 10.1128/jvi.4.6.890-895.1969

Role of Lysine in the Replication of Reovirus

I. Synthesis of Complete and Empty Virions

Philip C Loh 1, Herbert K Oie 1
PMCID: PMC375953  PMID: 16789122

Abstract

Lysine is essential for the replication of infectious reovirus. Omission of lysine from the extracellular medium not only permitted the continued synthesis of structural viral proteins and viral double-stranded ribonucleic acid (RNA), but also caused an enhanced formation of viral structures which were separable by isopycnic sedimentation of CsCl into a top band consisting of empty particles with a buoyant density of 1.29 g/cm3 and essentially free of viral RNA, and two lower bands which were difficult to resolve and had an average buoyant density of 1.37 g/cm3. The lower bands contained most of the viral nucleic acid. The above effects were reversed when lysine was restored early after infection. In contrast, a single band with a buoyant density of 1.38 g/cm3 was obtained from lysine-plus infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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