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. 1970 Jan;5(1):14–21. doi: 10.1128/jvi.5.1.14-21.1970

Virus-Specific Antigens in Hamster Cells Transformed by Rous Sarcoma Virus

Erwin Fleissner 1
PMCID: PMC375963  PMID: 4315157

Abstract

Virus-specific antigens were studied in hamster cells transformed by Rous sarcoma virus (RSV). Antigens were localized in the cytoplasm, as demonstrated by fluorescent antibody staining of fixed cells as well as by complement fixation (CF) following subcellular fractionation. Cytoplasmic extracts were analyzed by velocity and isopycnic centrifugation. CF antigens were found in a soluble form and in association with membranes and polyribosomes. Isolated plasma membranes had no CF antigen. Both soluble and particulate fractions with CF activity contained the same antigenic determinants by Ouchterlony analysis. These antigenic determinants were identical to those released by ether treatment of RSV.

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Selected References

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