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. 1970 Feb;5(2):153–164. doi: 10.1128/jvi.5.2.153-164.1970

Developmental Sequence and Intracellular Sites of Synthesis of Three Structural Protein Antigens of Influenza A2 Virus

Koichiro Maeno 1,1, Edwin D Kilbourne 1
PMCID: PMC375981  PMID: 4914627

Abstract

Specific antisera for hemagglutinin (HA) and neuraminidase antigens of influenza A2 virus (A2E) were produced through the segregation of the two proteins in reciprocal viral recombinants of A2E and A0e viruses. Gamma globulin fractions of these specific antisera and of antiserum specific for the nucleoprotein (NP) antigen of A0e virus were conjugated with fluorescein isothiocyanate and employed to follow the synthesis of the three structural proteins in clone 1-5C-4 human aneuploid cells, with parallel measurement of serological and biological activity of the antigens by other techniques. In this system, NP antigen appeared first (at 3 hr) in the cell nucleus, whereas HA and neuraminidase appeared coincidentally, at 4 hr after infection, in the cytoplasm. The initial detectability of biological or complement-fixing activity of the proteins coincided with their demonstrability as stainable antigens. Late in infection, all three antigens were detected at the cell surface. Antibody specific for HA partially blocked the intracellular staining of neuraminidase and inhibited the enzymatic activity of both extracted and intact extracellular virus. These observations suggest the close intracytoplasmic proximity of the two envelope antigens and perhaps their initial association in a larger protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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