Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1970 Mar;5(3):309–312. doi: 10.1128/jvi.5.3.309-312.1970

Uncoating of Poliovirus by Isolated Plasma Membranes

V F Chan 1,1, F L Black 1
PMCID: PMC376007  PMID: 4314552

Abstract

Poliovirus type I LSc strain labeled with 14C-uridine was adsorbed onto isolated plasma membranes and incubated with them. When membranes from Hep-2 or Vero cells were used, 22% of the label was converted to a trichloroacetic acid-soluble form, when trypsin or ribonuclease was added, the fraction rendered soluble was increased, and when the two enzymes were added in sequence, 85% or more of the label became trichloroacetic acid-soluble. This labilization of poliovirus could be reproduced when butanol-solubilized proteins from membranes were substituted for the whole plasma membranes, but it did not occur with membranes from polio-virus-resistant calf kidney or BHK-21 cells.

Full text

PDF
309

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billeter M. A., Weissmann C., Warner R. C. Replication of viral ribonucleic acid. IX. Properties of double-stranded RNA from Escherichia coli infected with bacteriophage MS2. J Mol Biol. 1966 May;17(1):145–173. doi: 10.1016/s0022-2836(66)80101-8. [DOI] [PubMed] [Google Scholar]
  2. HOLLAND J. J., HOYER B. H. Early stages of enterovirus infection. Cold Spring Harb Symp Quant Biol. 1962;27:101–112. doi: 10.1101/sqb.1962.027.001.013. [DOI] [PubMed] [Google Scholar]
  3. HOLLAND J. J. Irreversible eclipse of poliovirus by HeLa cells. Virology. 1962 Feb;16:163–176. doi: 10.1016/0042-6822(62)90292-1. [DOI] [PubMed] [Google Scholar]
  4. HOLLAND J. J., McLAREN L. C. The location and nature of enterovirus receptors in susceptible cells. J Exp Med. 1961 Aug 1;114:161–171. doi: 10.1084/jem.114.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOLLAND J. J., McLAREN L. C. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959 May 1;109(5):487–504. doi: 10.1084/jem.109.5.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOKLIK W. K., DARNELL J. E., Jr The adsorption and early fate of purified poliovirus in HeLa cells. Virology. 1961 Apr;13:439–447. doi: 10.1016/0042-6822(61)90275-6. [DOI] [PubMed] [Google Scholar]
  7. Mandel B. The relationship between penetration and uncoating of poliovirus in HeLa cells. Virology. 1967 Apr;31(4):702–712. doi: 10.1016/0042-6822(67)90198-5. [DOI] [PubMed] [Google Scholar]
  8. PHILIPSON L., LIND M. ENTEROVIRUS ECLIPSE IN A CELL-FREE SYSTEM. Virology. 1964 Jul;23:322–332. doi: 10.1016/0042-6822(64)90254-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES