Abstract
Evidence is presented that the entire enterovirus ribonucleic acid genome is translated into a single giant polypeptide of well over 200,000 daltons molecular weight. This giant protein was detected repeatedly in coxsackievirus B1-infected cells, even in the absence of amino acid analogues. The enzymes which cleave this giant protein into smaller structural and enzymatic proteins accumulate with increasing time after infection. It appears that they are either virus-coded proteolytic enzymes or else host enzymes which are activated or released from an intracellular site as a result of virus infection. These cleavage enzymes do not cause grossly apparent cleavage of host-cell proteins.
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Selected References
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- Buck C. A., Granger G. A., Taylor M. W., Holland J. J. Efficient, inefficient, and abortive infection of different mammalian cells by small RNA viruses. Virology. 1967 Sep;33(1):36–46. doi: 10.1016/0042-6822(67)90091-8. [DOI] [PubMed] [Google Scholar]
- Holland J. J., Kiehn E. D. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015–1022. doi: 10.1073/pnas.60.3.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holland J. J. Virus-directed protein synthesis in different animal and human cells. Science. 1968 Jun 21;160(3834):1346–1348. doi: 10.1126/science.160.3834.1346. [DOI] [PubMed] [Google Scholar]
- Jacobson M. F., Baltimore D. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein. J Mol Biol. 1968 Apr 28;33(2):369–378. doi: 10.1016/0022-2836(68)90195-2. [DOI] [PubMed] [Google Scholar]
- Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
- Maizel J. V., Jr, Summers D. F. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells. Virology. 1968 Sep;36(1):48–54. doi: 10.1016/0042-6822(68)90115-3. [DOI] [PubMed] [Google Scholar]
- Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr Evidence for large precursor proteins in poliovirus synthesis. Proc Natl Acad Sci U S A. 1968 Mar;59(3):966–971. doi: 10.1073/pnas.59.3.966. [DOI] [PMC free article] [PubMed] [Google Scholar]