Identifying permethrin resistance loci in malaria vectors by genetic mapping

SUMMARY

Identification of the major loci responsible for insecticide resistance in malaria vectors would aid the development and implementation of effective resistance management strategies, which are urgently needed to tackle the growing threat posed by resistance to the limited insecticides available for malaria control. Genome-wide association studies in the major malaria vector, Anopheles gambiae, have been hindered by the high degree of within-population structuring and very low levels of linkage disequilibrium hence we revisited the use of quantitative trait loci (QTL) mapping to study resistance phenotypes in this vector species. Earlier work, identified two major QTL associated with pyrethroid resistance in A. gambiae s.s. from East Africa using genetic crossing of laboratory-colonized resistant and susceptible strains. In this study, we report the results from genetic mapping of pyrethroid resistance in three isofemale pedigrees established from wild-caught female A. gambiae s.s. mosquitoes from Benin. We identified two QTL on chromosomes 2L and 3R in these field populations, in similar genomic locations to the QTL identified in laboratory strains. The relative merits of two alternative study designs are discussed and suggestions made for future genetic mapping studies of insecticide resistance in mosquitoes.

INTRODUCTION

Despite extensive control efforts, over half the world's population remain at risk of malaria and the disease has a massive impact on health and economic development, particularly in Africa (WHO, 2011). The main vector control methods, indoor residual spraying (IRS) and insecticide-treated bed nets (ITNs), rely on insecticides but resistance to the limited number of available insecticides is developing rapidly, with some populations now showing resistance to all four classes (Ranson et al. 2009; Constant et al. 2012). Resistance to pyrethroid insecticides is of greatest concern as these are the major class of insecticides used in public health and the only insecticide class permitted for impregnation of mosquito nets (WHO, 2012). If pyrethroid insecticides were to fail due to resistance of target populations, WHO estimates a loss of ∼50% of the current impact on vector control in Africa (WHO, 2012).

Attempts to identify the genetic basis of pyrethroid resistance in malaria vectors have focused largely on genotyping for known target site mutations and/or comparing transcript levels in insecticide-resistant and -susceptible populations using microarrays. Two alternative amino acid substitutions in the Anopheles gambiae voltage-gated sodium channel, L1014F and L1014S, have been clearly associated with resistance to pyrethroids (Martinez-Torres et al. 1998; Ranson et al. 2000a; Reimer et al. 2008). However, the recent identification of an additional mutation (N1575Y), in a distinct part of the sodium channel protein which confers additional protection against insecticide exposure (Jones et al. 2012), highlights the potential pitfalls of relying on screening single loci. Gene expression microarray-based approaches in A. gambiae have evolved from small custom-made arrays containing probes for gene families associated with insecticide detoxification (David et al. 2005) to whole genome arrays (Mitchell et al. 2012). These approaches have successfully identified overexpressed candidates, several of which have subsequently been shown to metabolize pyrethroids (Müller et al. 2008; Stevenson et al. 2011; Mitchell et al. 2012). However microarray data sets do not lead directly to the identification of resistance-associated polymorphisms in DNA and extensive follow-up work is often required to identify these loci.

The sequencing of the A. gambiae genome (Holt et al. 2002; Lawniczak et al. 2010) has identified a large number of single nucleotide polymorphisms (SNPs) which have enabled association-mapping techniques to be applied to identify resistance loci. As with the microarray-based approaches, these studies initially relied on using SNPs within, or in the vicinity, of known candidate loci (Weetman et al. 2010) but more recently have progressed to genome-wide association studies (GWAS). However, the low levels of linkage disequilibrium and high levels of population substructuring (Neafsey et al. 2010; Weetman et al. 2010), have limited the utility of these approaches in A. gambiae and efforts are now being directed to using next generation sequencing to study this trait (Weetman, Neafsey and Donnelly, personal communication).

Early studies using genetic mapping to identify insecticide resistance-associated loci were reliant on morphological markers. In the 1960s, DDT resistance was reported to be a monofactorial trait in several insect species and linked to the brown body marker in the housefly, Musca domestica (Milani, 1963). In the 1990s, genetic evidence of close association between the kdr phenotype and the voltage-gated sodium channel was established (Williamson et al. 1993; Knipple et al. 1994). The genetic basis of non-target site resistance has proved less tractable to study. Studies in the housefly identified up to nine individual resistance loci, controlling traits such as insecticide metabolism and penetration. The co-localization of the majority of these on a single chromosome led to the suggestion that there is a major regulatory locus that controls multiple resistance mechanisms (Plapp, 1976). Genetic linkage maps greatly improved with the advent of techniques for screening molecular markers such as microsatellites and Restriction Fragment Length Polymorphisms (RFLP) (Heckel, 1993). This facilitated the resolution of quantitative trait loci (QTL) controlling polygenic traits.

The first QTL to be identified in A. gambiae, were associated with refractoriness to Plasmodium parasites (Zheng et al. 1997). Later, similar approaches were used to identify QTL associated with permethrin or DDT resistance in laboratory strains of A. gambiae (Ranson et al. 2000b, 2004), Anopheles funestus (Wondji et al. 2007b), Anopheles arabiensis (Witzig et al. 2013) and Aedes aegypti (Saavedra-Rodriguez et al. 2008). These studies have in common, that they used experimental crosses between a resistant and a susceptible population to generate mapping populations. However, it is not clear whether the loci identified in laboratory-colonized strains are truly representative of those found in natural populations. In an alternative approach, natural pedigrees, collected from field-caught females, are used to map allelic variants affecting parasite development (Niare et al. 2002; Riehle et al. 2006). Here we have adopted the latter approach to map the major QTL associated with resistance to permethrin in A. gambiae in West Africa.

Benin was chosen as a study site due to the high level of pyrethroid resistance in the south of the country (Djègbè et al. 2011) where A. gambiae s.s. is the major malaria vector. A microarray gene expression study on a permethrin resistant population from Akron, an urban agricultural site on the outskirts of the capital city Porto-Novo, identified several candidate genes associated with resistance (Djouaka et al. 2008). These included the cytochrome P450s Cyp6p3 and Cyp6m2 that have previously been associated with pyrethroid resistance (Müller et al. 2008; Stevenson et al. 2011) and two cuticular precursor genes (Cplcg3 and Cplcg4). These cuticular genes were also found to be overexpressed in permethrin resistant A. gambiae samples from neighbouring Nigeria (Awolola et al. 2009). The 1014F kdr allele is also found at a high frequency of >86% in A. gambiae s.s. from this area (Yadouleton et al. 2010).

MATERIALS AND METHODS

Field collections and molecular characterization

Gravid or blood-fed A. gambiae s.l. adult females resting indoors were collected in houses in Akron, Porto-Novo, Benin (lat: 4.4300; long: 2.6400) between 6–8 am or during the night from 12 midnight to 6 am in September 2009. Live Anopheles mosquitoes were transferred to cages and blood fed on two or three subsequent nights on live guinea pigs to ensure all females could feed to satiation. Four to six days after blood feeding individual gravid females were transferred into 1·5 ml Eppendorf tubes for egg laying (Morgan et al. 2010). After oviposition the females (F₀) were separated from eggs and stored over silica gel for later molecular analysis. Eggs were transported to the Liverpool School of Tropical Medicine, and families were raised to adults separately.

Field-collected females were morphologically identified as belonging to the A. gambiae species complex according to the key of (Gillies and de Meillon, 1968) and identified as A. gambiae s.s. M-form by PCR (Santolamazza et al. 2008). F₁ progeny from individual isofemale lines were inbred to form an F₂ generation which was then phenotyped for permethrin resistance as described below.

DNA was extracted from the parental females (F₀) eight F₁ progeny from each family (four males, four females), and all F₂ progeny using the LIVAK method (Collins et al. 1987). The F₀ and F₁ individuals were genotyped for the 1014F/S kdr and 119S ace-1 (mutation in the sequence for acetylcholinesterase conferring target-site resistance to carbamate insecticides) alleles using a pyrosequencing assay (Witzig et al. 2013). The kdr genotype was additionally confirmed through sequence analysis for the three parental females. Karyotypes for the 2La inversion (White et al. 2007) and the 2Rb inversions (Lobo et al. 2010) were determined by PCR for each family in F₀, eight F₁ samples and, for 2La only, in all F₂ offspring.

Insecticide susceptibility assays

To assess the resistance profile of the Akron population, adults (2–5 days old) reared from field-collected larvae were exposed to permethrin (0·75%), bendiocarb (0·1%) or DDT (4%) for one hour. Insecticide susceptibility assays were carried out following the WHO protocol (WHO, 1998). Mortality was determined after 24 h; lower and upper limits of CI 95% were calculated using VassarStats (Lowry, 2004). Dead and alive individuals were counted and stored separately over silica gel for DNA extraction.

For resistance phenotyping of F₂ offspring, 1–3 day old male and female F₂ progeny of three families were exposed to 0·75% permethrin (family 17) or 2% permethrin (families 15 and 25, due to their low mortality in initial bioassays with 0·75% permethrin) for one hour and scored as dead or alive 24 h after exposure and kept on silica gel for further molecular analysis.

Genotyping of mapping families

A subset of previously described microsatellite markers (Zheng et al. 1996; Ranson et al. 2004; Witzig et al. 2013) were selected to include markers on each chromosome arm. Microsatellite loci were initially screened using a tailed primer system described by (Oetting et al. 1998). Primer pairs for informative markers (Table 1 and S1 – the latter in Online version only) were re-synthesized to directly incorporate a fluorescent dye (WellRed oligos, Sigma-Aldrich). Each PCR was made by mixing 11 μl dd H₂O, 1·5 μl 10× Taq buffer (1·5 mm MgCl₂), 0·3 μl of 10 mm dNTPs, 0·3 μl of 25 mm MgCl₂, 0·5 μl each of 10 mm site specific forward and reverse primer, 0·12 μl of Taq Polymerase and 1 μl of gDNA to a final volume of 15 μl. Cycling conditions were: 95 °C/5 min; (95 °C/45 s; 57 °C/45 s; 72 °C/45 s) for 35 cycles; 72 °C/10 min. PCR products of up to three loci were combined and fragment analysis was performed on a CEQ™ 8000 (Beckman Coulter, Inc., USA). Microsatellite genotypes were scored using the GenomeLab GeXP software (version 10.0.9; Beckman Coulter).

Table 1.

Details of microsatellite and SNP markers used for genotyping the F₂ progeny of families 15, 17 and 25 including the physical distances in Mb according to VectorBase, http://www.vectorbase.org, A. gambiae PEST annotation, AgamP3.5 (Megy et al. 2012), and allele size range

	Marker	Source	Locationa (Mb)	Allele size range (bp)	15	17	25
X chromosome	53	1	3·6	87–97		x
	80	1	7·0	80–108		x
Chromosome 2	46	1	1·2	133–142			x
2R	803	1	1·9	144–146	x	x
	197	1	10·4	83–96	x	x
	757	1	14·7	79–101	x	x	x
	799	1	24·9	74–93			x
	2R_si_5	3	28·5	246–256	x	x	x
	SNP1b		28·5		x	x	x
	770	1	40·4	174–188		x	x
	117	1	42·7	116–123		x	x
2L	kdrb		24·2			x
	637	1	25·5	102–105	x
	787	1	26·8	131–135		x	x
	772	1	42·0	131–152			x
	603	1	42·0	105–109		x
	675	1	46·9	101–114	x		x
Chromosome 3	776	1	2·5	87–102	x	x	x
3R	59	1	4·2	120–132	x	x	x
	812	1	6·2	107–131	x	x	x
	SNP2b		6·9			x	x
	249	1	8·8	108–132	x	x	x
	30C1	2	9·6	155–168	x	x	x
	SNP3b		10·9		x	x	x
	06M19	2	13·1	160–170	x	x	x
	119	1	14·8	179–194	x		x
	555	1	21·3	83–96	x
	158	1	22·99	78–90			x
	341	1	23·02	116–142		x	x
	06H01	2	25·5	127–156	x	x	x
	33C1	2	25·6	147–159		x
	06F05	2	31·6	174–184		x	x
	88	1	34·7	184–197			x
	30L17	2	41·1	71–79		x	x
3L	SODI (RFLP)b		5·6			x
	811	1	14·8	124–134		x	x
	577	1	19·8	80–103	x	x
	817	1	31·9	143–149		x	x
	46C2	2	41·4	177–197		x	x
			Sum Chromosome x		0	2	0
			Sum Chromosome 2		7	10	10
			Sum Chromosome 3		11	18	18
				Sum	18	30	28

^aAccording to vector base (08/2010).

^bSNP markers.

Source: 1, Zheng et al. (1996); 2, Ranson et al. (2004); 3, Witzig et al. (2013).

Three SNP markers (one on 2R, and two on 3R) were identified by de novo sequence analysis using the primers shown in Table S1 – in Online version only. Pyrosequencing assays were designed to score SNPs in each of these genes (AGAP002865, AGAP008212 and AGAP008447) (Table S2 – in Online version only). In addition, the 1014 kdr genotype in the F₂ progeny was determined by pyrosequencing as described previously (Wondji et al. 2007a; Witzig et al. 2013).

Marker data analysis, linkage mapping and LOD scoring

Associations between genotypes at each locus and the resistance phenotype (dead or alive) were assessed. Single marker analysis was performed for each family to test for association (P ⩽ 0·05) between genotype and resistance phenotype using Haploview 4.2 (Barrett et al. 2005). The JoinMap 2.0 software package (Stam and van Ooijen, 1996) was used to build genetic linkage maps for each family. Data from linkage maps along with the genotype/phenotype data were entered into Windows QTL Cartographer 2.5 (Wang et al. 2005) and interval mapping (IM) (Lander and Botstein, 1989; Jansen, 1993), composite interval mapping (CIM) (Zeng, 1993) and multiple interval mapping (MIM) (Kao et al. 1999) procedures were performed for each family separately and for the combined data.

MIM uses multiple marker intervals simultaneously to fit multiple putative QTL directly in the model for mapping QTL. For MIM an initial model was created by forward and backward markers selection with a significance level of 0·05. The model was refined by optimizing QTL positions, searching for new and testing for existing QTLs.

RESULTS

Insecticide susceptibility assays

Insecticide bioassays confirmed the presence of DDT and permethrin resistance in the A. gambiae s.l. Akron population but full susceptibility to bendiocarb. Twenty four hour mortality in adults raised from field-collected larvae was 52% (n = 82, CI 95% lower limit 0·41, upper limit 0·63) to permethrin, 0% to DDT (n = 60, CI 95% lower limit 0, upper limit 0·075) and 100% to bendiocarb (n = 96, CI 95% lower limit 0·95, upper limit 1).

A total of 109 females were collected and 59% (64 females) laid eggs. Forty seven of these egg batches were fertile (hatching rate 73%). The progeny of 21 families were kept as separate families of approximately 15–40 F₁ individuals. The 1014F kdr frequency in wild-caught females was 0·92 (n = 74, CI 95% lower limit 0·86, upper limit 0·96). All females were M form and neither the G119S ace-1 mutation in the gene for acetylcholinesterase nor the 1014S or 1575Y mutations in the voltage-gated sodium channel (Ranson et al. 2000a; Jones et al. 2012) were detected.

Only three of the 21 families produced sufficient F₂ progeny for phenotyping. This drop in progeny numbers from F₁ to F₂ highlights the difficulties in colonizing wild-caught A. gambiae and, as discussed below, is an important limitation of the natural pedigree approach. All three families showed strong permethrin resistance. Families 15 and 25 were initially phenotyped using 0·75% permethrin (the WHO diagnostic dose) but 100% survived a one hour exposure and mortality rates ranging from 5·3% to 20% mortality were obtained after 90 min. Using a higher concentration of permethrin (2%) and an exposure time of 60 min resulted in a higher mortality of 46 and 51% for families 15 and 25 respectively (Table S3 – in Online version only). The total numbers of phenotyped individuals in those families ranged from 43 to 68 mosquitoes. Mortality in females was significantly lower (P < 0·05, Mann–Whitney U test) than mortality in males in two out of the three families which were exposed to the higher dose of permethrin (Table S3 – in Online version only).

Genotyping of mapping families

Informative markers were selected on the basis of the genotypes of the parental female F₀ and eight F₁ individuals. Informative markers are loci with variation in the genotyped alleles such that at least one of those alleles could be clearly tracked back to one (partially informative) or both (fully informative) of the two parents. Of the 51 microsatellites tested, 34 were fully or partially informative in at least one of the three families, with nine informative in all three families and 14 informative in two families. Details of the number of markers informative for each family are shown in Table 1.

Three SNPs were identified by de novo sequence analysis (Tables S2 and S3 – in Online version only). These were informative in all families, with the exception of the SNP2 in family 15. The 1014 kdr marker was only informative in family 17. No alternative SNP marker in the sodium channel was identified in the remaining families.

The three parental F₀ females were homozygous for 1014F kdr allele. All individuals in families 15 and 25 were homozygous for kdr (1014F) whereas individuals of family 17 contained both 1014F and 1014L alleles (f(1014F) F₁ = 0·19; f(1014F) F₂ = 0·35). The presence of both 1014F and 1014L alleles in F₁ of family 17 indicates that the male parent of this line was heterozygote for kdr (as the parental female was confirmed as homozygous for 1014F).

Inversions can interfere with genetic mapping. Hence each family was characterized for the presence of two common inversions, 2Rb and 2La. All F₀ and F₁ specimens were homozygous for the inverted 2Rb⁺ arrangement. Both the standard (S) and inverted (I) arrangements of the 2La inversion were present in the F₂ progeny. Families 15 and 17 show similar frequencies of the standard arrangement with f(S) = 0·49 (with χ² = 0·05 and P value = 0·82) and f(S) = 0·47 (with χ² = 0·58 and P value = 0·45) respectively. Family 25 shows values of f(S) = 0·35 and f(I) = 0·65 (with χ² = 1·32 and P value = 0·25).

Linkage mapping and QTL analysis

Initially separate linkage maps were generated for each family. Subsequently data from three families (Table 2) were used to build a genetic linkage map containing data from a total of 144 F₂ individuals at 76 loci (Fig. 2). The length of the map is 180·3 cM with an average resolution of 6·9 cM/marker. Only two informative markers were identified on the X chromosome and it was not possible to generate a linkage map for this chromosome.

Table 2.

Summary of F₂ progeny used for genetic mapping, including number of markers genotyped in each family

Family	Number of F2 offspring genotyped							Number of markers genotyped (chromosome X, 2, 3)
	Dead			Alive			Total
	Sum	♀	♂	Sum	♀	♂	Sum
15	19	6	13	19	6	13	38	18 (0, 7, 11)
17	16	6	10	47	17	30	63	30 (2, 10, 18)
25	21	15	6	22	9	13	43	28 (0, 10, 18)
Sum	56	27	29	88	32	56	144	76 (2, 27, 47)

Fig. 2.

LOD plots and genetic linkage maps using the combined data from the three families. The location of the voltage-gated sodium channel is indicated (VGS) based on its physical location between markers 117 and 787. A scale of genetic distance in centimorgans is shown on the left; the names of the genetic markers are given on the right. Plot of LOD scores estimated by IM (dashed) and CIM (solid) for all three families. The dotted line represents a LOD score of 2·5, typically used as a cut off for significance (Lander and Botstein, 1989).

The software Windows QTL cartographer version 2.5 (Wang et al. 2005) was applied to test for linkage between resistance phenotype and genotype using both IM and CIM. IM uses one marker interval at a time to construct a putative QTL for testing by performing a likelihood ratio test at every position in the interval. The IM approach considers one QTL per model, and this can cause problems when multiple QTL are present in one linkage group. CIM is a modification of IM in which additional markers are included as cofactors in the analysis in order to deal with multiple QTL problems (Zheng et al. 1996).

As each family had a different genetic background, and two families were phenotyped using a higher insecticide dose, the results from individual families were first analysed individually (Fig. S1 – in Online version only). One QTL was detected on chromosome 2L reaching a LOD value of 3·1 but only in family 25 and only when using CIM. A second QTL was identified on chromosome 3R in family 15. This QTL is detected by both IM and CIM although less pronounced by IM (LOD score of 2·5 vs 1·4). The presence of this chromosome 3 QTL in family 15 is supported by the single marker analysis which tests for significant associations between genotype and phenotype. This is plotted in Fig. 1 which shows mortality rate as a function of the number of alleles inherited from the resistant parent.

Fig. 1.

Mortality rate as a function of alleles inherited from the resistant parent (maternal) for markers on chromosome 3R for family 15 showing significant phenotype: genotype association.

The data for all three families were then combined and CIM and IM repeated. Two clear QTLs were readily identifiable using both methodologies (Fig. 2). The QTL on chromosome 2L and chromosome 3R had associated LOD scores of 2·5 and 2·0, respectively using CIM. MIM also predicted the two QTLs, which together account for 13·2% of the genotypic variance for the combined data set (Table 3).

Table 3.

MIM estimates of QTL position and associated genetic variance associated with permethrin survival QTL in A. gambiae

	σg2 [%]	σg2 [%] per locus	Nearest marker	¥	Genetic distance [cM]
Combined all families
	13·2	56	117*	2Ra	54·3
		44	249	3R	18·3

σg2 [%] genetic variance in % of overall estimated phenotypic variance and per locus.

¥ Chromosome and location in genetic distance for nearest marker.

^aAssociated marker located on chromosome 2R, but effect possibly due to kdr on 2L.

DISCUSSION

This study identified two potential QTL associated with permethrin resistance in M form A. gambiae s.s. from southern Benin. The first of these is located on chromosome 2 and spans the genomic region encoding the major pyrethroid target site, the voltage-gated sodium channel. Two of the three families were fixed for the 1014F kdr allele itself and no further SNPs to add additional genotyping information could be identified within this gene to enable the linkage between the target site and this QTL to be assessed. The vicinity of the centromere and the heterogeneity in the 2La karyotype, may have both contributed to a reduced recombination in this region of the genome. Finer scale mapping in this region was impeded by the lack of informative markers that were identified on chromosome 2L. Hence at this stage it can only be postulated that the chromosome 2 QTL equates to target site resistance.

The second putative QTL is located on chromosome 3R spanning chromosomal division 30A–D. Chromosome 3R is densely clustered with detoxification genes and 18 P450 genes are located within the boundaries of this QTL, including Cyp6m2, identified as a candidate resistance gene in microarray experiments (Djouaka et al. 2008; Müller et al. 2008; Mitchell et al. 2012) and Cyp6z1 and Cyp6z2, which have been found associated with pyrethroid resistance in other colonies (Nikou et al. 2003; David et al. 2005; Müller et al. 2007). In addition 12 of the 27 Cplcg genes (cuticular proteins with low-sequence complexity) in A. gambiae are found clustered within division 30D on chromosome 3R (Willis, 2010). Overexpression of cuticular proteins causing reduced cuticular penetration of insecticides has been implicated in insecticide resistance (Puinean et al. 2010). Two of the genes in this cluster, Cplcg3 and Cplcg4, were found over expressed in resistant populations from southern Benin (Djouaka et al. 2008). Determining the precise nature of the chromosome 3R QTL will require fine-scale mapping on advanced intercross lines followed by functional characterization of candidate genes (Wondji et al. 2009).

QTL analysis of the X chromosome was not possible as only two informative markers were identified on this chromosome. Based on a smaller effective population size the average level of polymorphism on the X chromosome is reduced (Holt et al. 2002) making identification of informative markers very difficult. The absence of a QTL on 2R around the candidate gene Cyp6p3 which was previously found to be highly overexpressed in this population could be due to the limitations in the study, such as small number of families tested in this experiment or due to the lack of sufficient markers around this region. Alternatively, it may indicate that over expression of this P450 is under the control of a trans regulator.

Interestingly, there are parallels between the results of the current study, and previous QTL mapping studies of permethrin resistance using laboratory colonies originating from East Africa (Ranson et al. 2004). Both studies identified two QTL with similar boundaries, and in both the chromosome 3R QTL spans chromosomal divisions 30A–30C, encompassing several large clusters of P450 genes. However, given the inherent imprecision in genetic mapping studies on F₂ generations, it would be premature to conclude that the QTL identified in the Kenyan and Benin study equate to the same genetic factor.

Limitations of the study design

The current study was limited in its ability to precisely map QTL by the lack of informative markers, the numbers of available families and progeny, and difficulties in scoring the phenotype.

Heterogeneity at the 2La inversion may have restricted the mapping resolution in this region by suppressing recombination (Butlin, 2005). Previous genetic mapping studies on permethrin resistance in A. gambiae used a laboratory strain (RSP-ST) fixed for the 2La standard chromosomal arrangement to overcome this limitation (Ranson et al. 2004). However, it is also possible that variation was restricted by selective sweep surrounding the kdr locus, as has been detected in outbred wild populations (Lynd et al. 2010). The 1014F kdr locus is present at very high frequency in Southern Benin, and five of the six parents from the three isofemale lines used in the current study were fixed for this resistance allele reducing the variation among families in this part of chromosome 2.

To improve the study design presented, increasing the size of the mapping populations, either by using advanced intercross lines to increase both the sample size and number of recombination events is recommended. Pooling data of single families increased the mapping resolution of the QTL study only marginally from 7·1 to 6·9 cM/marker. But pooling may not always be advisable as there is a danger that it may mask rare QTLs. Increasing the number of families analysed may be advantageous, yet more families will not replace the need for a larger sample size within analysed families.

Further limitations were imposed by the phenotyping method adopted. Insecticide resistance is a quantitative trait yet by scoring only two outcomes after exposure to insecticides, alive or dead, the quantitative data are reduced to a binary trait. Scoring extreme phenotypes only, by using a range of exposure times, would have increased the power but still not converted this to a quantitative measurement. Other approaches, such as recording time to kill, were discarded as insecticides may not result in immediate lethality.

All previous QTL mapping studies in malaria vectors have utilized experimental crosses between two inbred laboratory strains of mosquitoes, a resistant and a susceptible population (Ranson et al. 2000b, 2004; Wondji et al. 2007b, 2009; Saavedra-Rodriguez et al. 2008) and usually these strains have been maintained under laboratory insecticide selection pressure for multiple generations prior to the establishment of the genetic crosses. Although this has several logistical advantages over the use of natural pedigrees, foremost of which is the ease with which additional families can be added to the study to enhance the resolution, there are also some considerable limitations. Some resistance alleles may prove unstable under laboratory conditions, being rapidly lost in colonies. This may be attributable to fitness costs associated with these resistance alleles or may simply be a result of genetic drift caused by bottlenecks that inevitably occur when adapting a mosquito population to insectary conditions. Furthermore, maintaining a laboratory colony under selection with insecticides may result in a phenotype that differs greatly from that found in natural populations. Theory suggests that laboratory selection for resistance preferentially selects for resistance factors of minor effects such as body size and developmental rate whereas selection in the field, which generally involves exposure to much higher concentrations, favours the selection of rare mutations with major effect (Ffrench-Constant et al. 2004). As a result, QTL studies using this design may be trying to establish genotype–phenotype associations based on a genetic make-up that is very unlikely to exist in field populations (Dworkin et al. 2005). A hybrid of these two approaches has recently been applied to identify the major loci associated with pyrethroid resistance in A. arabiensis from Chad. In this experiment, the mapping population was generated by crossing a laboratory susceptible colony with the F₁ generation from field collections of a resistant population. Establishing laboratory crosses reduced the restrictions imposed by limited fecundity and/or survivorship of natural pedigrees but also minimized the danger of loss of resistance alleles and/or selection or atypical alleles that results from prolonged laboratory colonization and selection (Witzig et al. 2013).

CONCLUSION

This study indicates the presence of two QTL associated with resistance to permethrin in natural pedigrees of A. gambiae M form from Benin. Similarities were identified with the QTLs detected in a laboratory pyrethroid-resistant strain originating from Kenya but until the boundaries of these QTL can be reduced it is premature to speculate whether they do indeed map to the same genetic factors. Intriguingly, results from microarray comparisons of transcripts overexpressed in pyrethroid-resistant and -susceptible strains across Africa are converging on a small number of candidate cytochrome P450 genes. Whether the QTLs identified in the mapping studies are responsible for oxidase based metabolic resistance in A. gambiae is currently unknown. Ongoing studies involving next generation sequencing of multiple pyrethroid resistant A. gambiae populations from across Africa may help to address this question.

Supplementary Material

Supplementary Material

Supplementary information supplied by authors.

FINANCIAL SUPPORT

C.S.W. is supported by a Wellcome Trust Research Career Development Fellowship.