Abstract
Cultures of L cells were synchronized with respect to deoxyribonucleic acid (DNA) synthesis with thymidine and 5-fluoro-2′-deoxyuridine (FUdR) and infected with Newcastle disease virus (NDV), mengovirus, or reovirus 3. Inhibition of incorporation of 3H-cytidine into the DNA of synchronized cells is partially inhibited 2 hr after infection with NDV or mengovirus and nearly completely suppressed 4 hr after infection. With NDV and mengovirus, no evidence was obtained of differences in sensitivity of cells during early S phase as compared to later stages in DNA synthesis. When cells were infected with reovirus at the time of release from FUdR block, inhibition of cellular DNA synthesis was evident at 2 to 3 hr, and it was complete at 4 to 5 hr after infection. However, when cells were infected several hours prerelease, synthesis of DNA occurred in early S phase in spite of the fact that the cells had been infected for up to 6 hr. The results indicate that DNA synthesis in early S phase is relatively insensitive to the inhibitory function of reovirus. Colorimetric determinations (diphenylamine reaction) of the amounts of DNA produced in synchronized cells have substantiated the inhibition of DNA synthesis observed by isotope incorporation techniques.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Comings D. E., Kakefuda T. Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968 Apr 14;33(1):225–229. doi: 10.1016/0022-2836(68)90290-8. [DOI] [PubMed] [Google Scholar]
- Ensminger W. D., Tamm I. Cellular DNA and protein synthesis in reovirus-infected L cells. Virology. 1969 Oct;39(2):357–360. doi: 10.1016/0042-6822(69)90062-2. [DOI] [PubMed] [Google Scholar]
- Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by Newcastle disease or mengovirus infection. Virology. 1970 Jan;40(1):152–165. doi: 10.1016/0042-6822(70)90387-9. [DOI] [PubMed] [Google Scholar]
- Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by reovirus infection. Virology. 1969 Dec;39(4):935–938. doi: 10.1016/0042-6822(69)90032-4. [DOI] [PubMed] [Google Scholar]
- FRANKLIN R. M., BALTIMORE D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harb Symp Quant Biol. 1962;27:175–198. doi: 10.1101/sqb.1962.027.001.019. [DOI] [PubMed] [Google Scholar]
- GOMATOS P. J., TAMM I. MACROMOLECULAR SYNTHESIS IN REOVIRUS-INFECTED L CELLS. Biochim Biophys Acta. 1963 Aug 20;72:651–653. [PubMed] [Google Scholar]
- Galavazi G., Schenk H., Bootsma D. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. I. Optimal conditions. Exp Cell Res. 1966 Feb;41(2):428–437. doi: 10.1016/s0014-4827(66)80149-0. [DOI] [PubMed] [Google Scholar]
- HUTCHISON W. C., MUNRO H. N. The determination of nucleic acids in biological materials. A review. Analyst. 1961 Dec;86:768–813. doi: 10.1039/an9618600768. [DOI] [PubMed] [Google Scholar]
- RUECKERT R. R., MUELLER G. C. Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency. Cancer Res. 1960 Dec;20:1584–1591. [PubMed] [Google Scholar]
- WHEELOCK E. F., TAMM I. Biochemical basis for alterations in structure and function of HeLa cells infected with Newcastle disease virus. J Exp Med. 1961 Nov 1;114:617–632. doi: 10.1084/jem.114.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]