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. 1970 Jun;5(6):718–725. doi: 10.1128/jvi.5.6.718-725.1970

Effect of the “Ribonucleic Acid Control” Locus in Escherichia coli on T4 Bacteriophage-Specific Ribonucleic Acid Synthesis

Ola Sköld 1
PMCID: PMC376066  PMID: 4914097

Abstract

Amino acid control of ribonucleic acid (RNA) synthesis in bacteria is known to be governed genetically by the rel locus. We investigated whether the rel gene of the host would also exert its effect on the regulation of phage-specific RNA synthesis in T4 phage-infected Escherichia coli cells. Since T-even phage infection completely shuts off host macromolecular synthesis, phage RNA synthesis could be followed specifically by the cumulative incorporation of radioactivity from labeled precursors into RNA of infected cells. Labeled uracil was shown to accumulate in phage-specific RNA for 30 to 35 min after infection, a phenomenon which probably reflects an expansion of the labile phage-RNA pool. Amino acid starvation was effected by the use of auxotrophic bacterial strains or thienylalanine. The latter substance is an amino acid analogue which induces a chemical auxotrophy by inhibiting the biosynthesis of phenylalanine, tyrosine, and tryptophan. Phage RNA synthesis was strictly dependent on the presence of amino acids, whereas phage deoxyribonucleic acid synthesis was not. By the use of several pairs of bacterial strains which were isogenic except for the rel gene, it was demonstrated that amino acid dependence was related to the allelic state of this gene. If the rel gene was mutated, amino acid starvation did not restrict phage RNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: requirements for late messenger synthesis. J Mol Biol. 1968 Apr 28;33(2):339–362. doi: 10.1016/0022-2836(68)90193-9. [DOI] [PubMed] [Google Scholar]
  2. EZEKIEL D. H. FALSE FEEDBACK INHIBITION OF AROMATIC AMINO ACID BIOSYNTHESIS BY BETA-2-THIENYLALANINE. Biochim Biophys Acta. 1965 Jan 11;95:54–62. doi: 10.1016/0005-2787(65)90210-8. [DOI] [PubMed] [Google Scholar]
  3. Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edlin G., Neuhard J. Regulation of nucleoside triphosphate pools in Escherichia coli. J Mol Biol. 1967 Mar 14;24(2):225–230. doi: 10.1016/0022-2836(67)90328-2. [DOI] [PubMed] [Google Scholar]
  5. Edlin G., Stent G. S., Baker R. F., Yanofsky C. Synthesis of a specific messenger RNA during amino acid starvation of Escherichia coli. J Mol Biol. 1968 Oct 28;37(2):257–268. doi: 10.1016/0022-2836(68)90266-0. [DOI] [PubMed] [Google Scholar]
  6. Fiil N., Friesen J. D. Isolation of "relaxed" mutants of Escherichia coli. J Bacteriol. 1968 Feb;95(2):729–731. doi: 10.1128/jb.95.2.729-731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lavallé R., De Hauwer G. Messenger RNA synthesis during amino acid starvation in Escherichia coli. J Mol Biol. 1968 Oct 28;37(2):269–288. doi: 10.1016/0022-2836(68)90267-2. [DOI] [PubMed] [Google Scholar]
  8. Morris D. W., Kjeldgaard N. O. Evidence for the non-co-ordinate regulation of ribonucleic acid synthesis in stringent strains of Escherichia coli. J Mol Biol. 1968 Jan 14;31(1):145–148. doi: 10.1016/0022-2836(68)90064-8. [DOI] [PubMed] [Google Scholar]
  9. NOMURA M., OKAMOTO K., ASANO K. RNA metabolism in Escherichia coli infected with bacteriophage T4. Inhibition of host ribosomal and soluble RNA synthesis by phage and effect of chloromycetin. J Mol Biol. 1962 May;4:376–387. doi: 10.1016/s0022-2836(62)80018-7. [DOI] [PubMed] [Google Scholar]
  10. Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nierlich D. P. Amino acid control over RNA synthesis: a re-evaluation. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345–1352. doi: 10.1073/pnas.60.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nierlich D. P. Radioisotope uptake as a measure of synthesis of messenger RNA. Science. 1967 Dec 1;158(3805):1186–1188. doi: 10.1126/science.158.3805.1186. [DOI] [PubMed] [Google Scholar]
  13. Nomura M., Witten C., Mantei N., Echols H. Inhibition of host nucleic acid synthesis by bacteriophage T4: effect of chloramphenicol at various multiplicities of infection. J Mol Biol. 1966 May;17(1):273–278. doi: 10.1016/s0022-2836(66)80107-9. [DOI] [PubMed] [Google Scholar]
  14. Olsnes S., Hauge J. G. Amino acid control of RNA synthesis in T4-infected Escherichia coli. Eur J Biochem. 1968 Dec;7(1):128–136. doi: 10.1111/j.1432-1033.1968.tb19583.x. [DOI] [PubMed] [Google Scholar]
  15. SKOELD O., BUCHANAN J. M. INHIBITION OF DEOXYRIBONUCLEIC ACID-DIRECTED RIBONUCLEIC ACID POLYMERASE IN ESCHERICHIA COLI AFTER INFECTION WITH BACTERIOPHAGE T4. Proc Natl Acad Sci U S A. 1964 Apr;51:553–560. doi: 10.1073/pnas.51.4.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shih A. Y., Eisenstadt J., Lengyel P. On the relation between ribonucleic acid synthesis and peptide chain initiation in E. coli. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1599–1605. doi: 10.1073/pnas.56.5.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Turnock G., Wild D. G. Phenotypic expression of the 'relaxed' genotype during inhibition of Escherichia coli by beta-2-thienylalanine. Biochem J. 1965 Aug;96(2):12C–14C. doi: 10.1042/bj0960012c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss S. B., Hsu W. T., Foft J. W., Scherberg N. H. Transfer RNA coded by the T4 bacteriophage genome. Proc Natl Acad Sci U S A. 1968 Sep;61(1):114–121. doi: 10.1073/pnas.61.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]

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