Abstract
The structure of Bacillus subtilis bacteriophage φ25 and φ25 deoxyribonucleic acid (DNA) were studied by electron microscopy. The head of φ25 is a regular polyhedron measuring 75 nm in diameter. The uncontracted tail of φ25 is 130 nm in length and includes a large, complex tail plate. Phage φ25 DNA is double-stranded and has a molecular weight of approximately 100 million as determined by electron microscopic length measurements and analytical band sedimentation in CsCl. The complementary strands of φ25 DNA contain numerous random interruptions. Chemical analysis of φ25 DNA demonstrated that 5-hydroxymethyluracil replaces thymine and that the DNA has a mole per cent (guanine plus cytosine) of 42.
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