Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1970 Jul;6(1):145–147. doi: 10.1128/jvi.6.1.145-147.1970

Molecular Weight of Sindbis Virus Ribonucleic Acid as Measured by Polyacrylamide Gel Electrophoresis

P Dobos 1,1, P Faulkner 1
PMCID: PMC376100  PMID: 5471476

Abstract

The molecular weight (MW) of Sindbis virion ribonucleic acid (RNA) determined by gel electrophoresis was in a range of 3.89 × 106 to 4.45 × 106 daltons. Upon denaturation with urea, it separated into fragments having an MW of 1.76 × 106 daltons. Viral-specific Sindbis 26S RNA contained a major species having an MW of 1.76 × 106 daltons.

Full text

PDF
145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Applebaum S. W., Ebstein R. P., Wyatt G. R. Dissociation of ribonucleic acid from silkmoth pupae by heat and dimethylsulfoxide: evidence for specific cleavage points. J Mol Biol. 1966 Oct 28;21(1):29–41. doi: 10.1016/0022-2836(66)90077-5. [DOI] [PubMed] [Google Scholar]
  2. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  3. Cartwright K. L., Burke D. Virus nucleic acids formed in chick embryo cells infected with Semliki Forest virus. J Gen Virol. 1970 Feb;6(2):231–248. doi: 10.1099/0022-1317-6-2-231. [DOI] [PubMed] [Google Scholar]
  4. Dobos P., Faulkner P. Characterization of a cytoplasmic fraction from Sindbis virus-infected cultures. Can J Microbiol. 1969 Feb;15(2):215–222. doi: 10.1139/m69-036. [DOI] [PubMed] [Google Scholar]
  5. Dobos P., Faulkner P. Properties of 42S and 26S Sindbis viral ribonucleic acid species. J Virol. 1969 Oct;4(4):429–438. doi: 10.1128/jvi.4.4.429-438.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  8. PFEFFERKORN E. R., HUNTER H. S. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS. Virology. 1963 Jul;20:433–445. doi: 10.1016/0042-6822(63)90092-8. [DOI] [PubMed] [Google Scholar]
  9. Richards E. G., Coll J. A., Gratzer W. B. Disc electrophoresis of ribonucleic acid in polyacrylamide gels. Anal Biochem. 1965 Sep;12(3):452–471. doi: 10.1016/0003-2697(65)90212-5. [DOI] [PubMed] [Google Scholar]
  10. Sprecher-Goldberger S. Differences between the structures of poliovirus and Sindbis virus infectious ribonucleic acids. Arch Gesamte Virusforsch. 1967;20(2):225–234. doi: 10.1007/BF01241276. [DOI] [PubMed] [Google Scholar]
  11. Sreevalsan T., Lockart R. Z., Jr, Dodson M. L., Jr, Hartman K. A. Replication of Western equine encephalomyelitis virus. I. Some chemical and physical characteristics of viral ribonucleic acid. J Virol. 1968 Jun;2(6):558–566. doi: 10.1128/jvi.2.6.558-566.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Strauss J. H., Jr, Burge B. W., Darnell J. E. Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology. 1969 Mar;37(3):367–376. doi: 10.1016/0042-6822(69)90220-7. [DOI] [PubMed] [Google Scholar]
  13. WECKER E. [Features of an infectious nucleic acid fraction from chick embryos infected with encephalitis virus. I. Physical and chemical featurs]. Z Naturforsch B. 1959 Jun;14B:370–378. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES